前列腺小体外泌蛋白检测在慢性前列腺炎诊断中的应用  

Application of Prostatic Exosomal Protein in the Diagnosis of Chronic Prostatitis

作　　者：赵寅生 易小敏 屠昌明 黄其峰 吴黎黎 贾健安 ZHAO Yin-sheng;YI Xiao-min;TU Chang-ming;HUANG Qi-feng;WU Li-li;JIA Jian-an(Departruent of Clinical Laboratory,901 Hospital of the Joint Logistics Support Force ofthe Chinese People’s Liberation Army,Hefei230031,China;Department of Urology,901 Hospital of the Joint Logistics Support Force ofthe Chinese People’s Liberation Army,Hefei230031,China)

机构地区：[1]中国人民解放军联勤保障部队第901医院检验科,合肥230031 [2]中国人民解放军联勤保障部队第901医院泌尿外科,合肥230031

出　　处：《现代检验医学杂志》2020年第2期9-11,共3页Journal of Modern Laboratory Medicine

基　　金：安徽省自然科学基金(编号:1708085QH204)。

摘　　要：目的探讨前列腺小体外泌蛋白(prostatic exosomal protein,PSEP)检测对慢性前列腺炎的诊断价值。方法收集102例慢性前列腺炎患者和70例健康体检者的尿液标本,应用前列腺小体外泌蛋白检测试剂盒,对患者尿液中PSEP进行定量检测,评价其诊断前列腺炎的灵敏度、特异度和临界值等指标,比较PSEP检测体系与临床诊断标准的差异。结果慢性前列腺炎患者PSEP检测结果为3.002(1.728~3.966)ng/ml,明显高于健康体检者的0.869(0.565~1.094)ng/ml,差异具有统计学意义(P<0.001)。根据受试者工作曲线计算PSEP的最佳临界值为1.238 ng/ml,ROC曲线下面积为0.922,对慢性前列腺炎的诊断敏感度为86.27%,特异度为90%。结论检测患者尿液中的PSEP,取材方便,结果可靠,能提高患者依从性及就诊舒服度,可作为临床上慢性前列腺炎诊断现有方法的补充,为慢性前列腺炎患者临床诊断提供了新的检测依据,值得向临床推广应用。Objective To investigate the diagnostic value of prostatic exosomal protein(PSEP) detection for chronic prostatitis. Methods A total of 102 patients with chronic prostatitis and 70 healthy subjects were enrolled and the urine wre collected. The PSEP test kit was used to quantitatively detect PSEP in urine. The sensitivity, specificity and Cut-off values were used to compare the differences between the PSEP detection system and clinical diagnostic criteria. Results The results of PSEP 3.002(1.728~3.966)ng/ml in patients with chronic prostatitis were significantly higher than those in healthy subjects, and the difference was statistically significant(P<0.001). The cut-off value of PSEP for diagnosis of chronic prostatitis was calculated to be 1.238 ng/ml according to the ROC curve, with the area under ROC curve, sensitivity and specificity were 0.922,86.27% and 90% respectively. Conclusion The detection of PSEP in the urine of patients is convenient and the results are more objective, urine PSEP detection could also improve the patient compliance and comfort levels. It can make up for the defects of clinical diagnosis for chronic prostatitis. Urine PSEP detection provides a novel and convenient diagnosis method for chronic prostatitis patients and is worthy of clinical application.

关 键 词：前列腺小体外泌蛋白 慢性前列腺炎 敏感度 特异度 ROC曲线 

分 类 号：R697.33[医药卫生—泌尿科学] R446.19[医药卫生—外科学]