巨幼红细胞性贫血患者血浆维生素B9和维生素B12水平对骨髓组织p53表达的影响研究  

Effects of Plasma Vitamin B9 and Vitamin B12 Levels on p53 Expression in Bone Marrow Tissues in Patients with Megaloblastic Anemia

作　　者：张蓉[1] 李国辉 王颖[1] 刘赞[1] 赵会迎 梁英民[1] ZHANG Rong;LI Guo-hui;WANG Ying;LIU Zan;ZHAO Hui-ying;LIANG Ying-min(Department of Blood,Xi’an High Tech Hospital,Xi’an 710065,China;Department of Blood,Tangdu Hospital of AirForce Military Medical University,Xi’an 710038,China)

机构地区：[1]西安高新医院血液科,西安710065 [2]空军军医大学唐都医院血液科,西安710038

出　　处：《现代检验医学杂志》2020年第2期12-14,21,共4页Journal of Modern Laboratory Medicine

基　　金：陕西省卫生计生科研基金项目(2016D062)。

摘　　要：目的探究巨幼红细胞性贫血(megaloblastic anemia, MBA)患者血浆维生素B9(VB9)和维生素B12(VB12)水平对p53表达的影响。方法选择自2015年3月~2018年5月68例MBA患者为病例组,另选取同期68例健康者为对照组。分别比较VB9和VB12的分布情况、比较p53在不同程度VB9和VB12分布中的表达水平、比较两组间同型半胱氨酸(Hcy)的含量和分布情况和分析低VB9和VB12的MBA患者同型半胱氨酸的含量与p53表达的相关性。结果病例组VB9和VB12水平分别为5.28±0.36ng/ml及144.51±28.17pg/ml,显著低于对照组(5.73±0.45ng/ml,176.33±31.54pg/ml),差异有统计学意义(t=-6.44,-6.20,均P<0.001)。在VB9和VB12含量均偏低时,病例组患者的分布频数显著高于对照组(t=4.847,P <0.05)。在VB9和VB12含量均偏低时,病例组细胞p53表达中度和重度染色的比例较高,与其余两组病例组的差异具有统计学意义(t=7.551,P <0.05)。病例组的同型半胱氨酸含量为43.2μmol/L,且同型半胱氨酸患者分布比例为80%,显著高于对照组(t=4.226,P <0.05)。在VB9和VB12含量均偏低时患者中同型半胱氨酸含量与p53表达呈正相关性(Y=0.655X+12.42,r=0.661)。结论 p53的高表达可作为低VB9和VB12水平诱导MBA产生的标志物,为MBA的诊断提供有效参考。Objective To explore the effect of plasma vitamin B9(VB9) and vitamin B12(VB12) levels on p53 expression in patients with megaloblastic anemia(MBA).Methods From March 2015 to May 2018, 68 MBA patients in Xi’an High Tech Hospital were selected as the case group, and 68 healthy patients in the same period were selected as the control group. The distribution of VB9 and VB12 was compared, the expression level of p53 in the distribution of VB9 and VB12 was compared, the content and distribution of homocysteine(Hcy) between the two groups were compared, and the correlation between the content of Hcy and the expression of p53 in MBA patients with low VB9 and VB12 was analyzed. Results The average levels of VB9 and VB12 in the case group were 5.28±0.36 ng/ml and 144.51±28.17 pg/ml, respectively, and significantly lower than that in the control group(5.73±0.45 ng/ml,176.33±31.54 pg/ml), the difference was statisticall significant(t=-6.44,-6.20,all P<0.001). When the contents of VB9 and VB12 were both low, the distribution frequency of patients in the case group was significantly higher than that in the control group(t=4.847, P<0.05). When the content of VB9 and VB12 were both low, the proportion of moderate and severe staining of p53 expression was the highest in the case group and the difference between the other two groups was statistically significant(t=7.551, P < 0.05). The content of Hcy in the case group was 43.2 mol/L and the distribution proportion of Hcy was 80%, which was significantly higher than that in the control group(t=4.226, P < 0.05). There was a positive linear association between Hcy content and p53 expression in patients with low levels of both VB9 and VB12(Y=0.655 X+12.42, r=0.661).Conclusion The high expression of p53 may serve as a marker for the induction of MBA with low VB9 and VB12 levels, providing an effective reference for the diagnosis of MBA.

关 键 词：巨幼红细胞性贫血 维生素B9 维生素B12 同型半胱氨酸 P53 

分 类 号：R556.2[医药卫生—血液循环系统疾病] R446.11[医药卫生—内科学]