严重脂血对Sysmex XE-2100全自动血液分析仪血细胞检测结果的影响  

Severel Pemia on Blood Routine Test Results of Sysmex XE-2100 Automatic Hematology Analyzer

作　　者：李寿敏 张茜 赵府 郭子微 惠维 王诚 LI Shou-min;ZHANG Qian;ZHAO Fu;GUO Zi-wei;HUI Wei;WANG Cheng(Department of Clinical Laboratory,Renmin Hospital of Liupanshui City,Guizhou Liupanshui 553001,China;Zunyi Medical University,Guizhou Zunyi 56300,China)

机构地区：[1]六盘水市人民医院检验科,贵州六盘水553001 [2]遵义医科大学,贵州遵义563000

出　　处：《现代检验医学杂志》2020年第2期100-103,共4页Journal of Modern Laboratory Medicine

摘　　要：目的研究严重脂血对Sysmex XE-2100全自动血液分析仪血细胞检测结果的影响。方法将30例无脂血、溶血、黄疸的标本进行血细胞检测及TG检测作为对照组。30例每份标本平均分两管,A,B两组标本分别吸出70,120μl血浆加入同等量的脂肪乳制备乳糜血。血细胞检测用Sysmex XE-2100血液分析仪检测,TG水平用Roche cobas-8000生化分析仪检测,然后根据脂血程度分组将检测结果与对照组进行比较。结果对于Sysmex XE-2100,A组和B组中Hb,MCH,MCHC,WBC,LYMPH%,MODO%,RDW-SD,RDW-CV水平与对照组相比,差异均有统计学意义(t=-20.722~15.005,均P <0.05)。B组中HCT,MCV,NEUT%水平与对照组相比,差异均有统计学意义(t=-5.829^-2.836,均P <0.05)。RBC,PLT,EOS%和BASO%水平在两个组中与对照组相比,差异无统计学意义(t=-0.054~1.229,均P> 0.05)。结论不同程度脂血会对Sysmex XE-2100血液分析仪检测指标产生不同的影响,首先受到影响的指标Hb,MCH,MCHC,WBC,LYMPH%,MONO%,RDW-SD和RDW-CV,当脂血程度达到更高时还会对HCT,MCV和NEUT%造成影响,同时会造成白细胞不分类的情况,RBC,PLT,EOS%和BASO%结果在本实验中不受脂血影响。Objective To investigate severe lipemia on blood routine test results of Sysmex XE-2100 automatic hematology analyzer. Methods 30 samples without lipemia, hemolysis and jaundice were served as the control group for blood routine and TG detection. Each sample was equally divided in 2 tubes and took one tube for making up one group. Group A,B respectively removed 70μl and 120μl plasma, respectively, than the isovolumetric lipid emulsion was added to prepare the lipemia sample. All samples were detected by using Sysmex XE-2100 hematology analyzer at the same time,the TG level was detected by the Roche cobas-8000 biochemical analyzer, and then the detected results of different degrees of lipemia samples were respectively compared with the results of corresponding samples in the control group. Results For Sysmex XE-2100, the level of Hb, MCH,MCHC,WBC,LIMPH%,MONO%,RDW-SD,RDW-CV in group A and group B, the difference was statitical significant(t=-20.722~15.005,all P < 0.05), the level of HCT, MCV, NEUT% in group B had statistical difference(t…=-5.829^-2.836,all P < 0.05) compared with those in the control group.The level of RBC,PLT,EOS%,BASO% in the two groups had no statistical difference(t=-0.054~1.229,all P > 0.05)compared with those in the control group. Conclusion Different degrees of lipid had different effects on the detection indicator of Sysmex XE-2100 hematology analyzer. First of all the affected indicators were Hb,MCH,MCHC,WBC, LYMPH%,MONO%,RDW-SD and RDW-CV,would also affect HCT, MCV and NEUT% when the degree of lipemia reaches higher. At the same time it would cause leukocyte disclassification. The results of RBC,PLT,EOS%,BASO% were not affected by lipemia in this experiment.

关 键 词：脂血 SYSMEX XE-2100 影响 

分 类 号：R446.111[医药卫生—诊断学]