油用牡丹黑斑病病原菌的分离鉴定  

Isolation and Identification of the Pathogen on Oilseed Peony Black Spot

作　　者：常雪 任爱芝[1] 张汕 杜鑫雅 袁凤英 赵培宝[1] 李多川[3] CHANG Xue;REN Ai-zhi;ZHANG Shan(Department of Plant Protection,Liaocheng University,Liaocheng,Shandong 252000;Liaocheng Forestry Bureau,Liaocheng,Shandong 252000)

机构地区：[1]聊城大学植物保护系,山东聊城252000 [2]聊城市林业局,山东聊城252000 [3]山东农业大学,山东泰安271018

出　　处：《安徽农业科学》2020年第9期161-163,171,共4页Journal of Anhui Agricultural Sciences

基　　金：山东省重点研发项目(2019GNC106018);山东省科技发展计划项目(2014GNC110020);山东省微生物重点实验室开放课题(SDKL2017015);大学生科技创新课题(CXCY2018070,cxcy2019y151)。

摘　　要：[目的]明确油用牡丹常见的黑斑病病原菌。[方法]通过组织分离法对油用牡丹黑斑病病原菌进行分离、纯化,按照柯赫氏法则进行致病性鉴定,并利用形态观察结合分子生物学手段对病原菌进行鉴定。[结果]油用牡丹黑斑病的病原菌为链格孢(Alternaria alternata)。[结论]该研究结果为油用牡丹病害综合防控奠定了基础。[Objective]To identify the pathogen of oilseed peony black spot.[Method]The pathogen of oilseed peony black spot was isolated and purified by tissue isolation method.The pathogenicity was proved by the Koch’s rule.The pathogen was identified based on morphological characteristics and molecular systematic.[Result]The pathogen of oilseed peony black spot was identified as Alternaria alternata.[Conclusion]The results of this study provided a foundation for the comprehensive prevention and control of oilseed peony diseases.

关 键 词：油用牡丹 黑斑病 病原菌 形态学鉴定 分子鉴定 

分 类 号：S432.4[农业科学—植物病理学]