濒危植物大花黄牡丹根际土壤中一株青霉菌的分离与鉴定  

Isolation and Identification of Penicillium from the Rhizosphere Soil of the Endangered Plant Paeonia ludlowii in Tibet

作　　者：禄亚洲 张二豪[2,3] 尹秀 蔡皓[2,3] 张盼 LU Ya-zhou;ZHANG Er-hao;YIN Xiu(Research Institute of Plateau Ecology,Tibet Agriculture and Animal Husbandry University,Nyingchi,Tibet 860000;Tibet Agriculture and Animal Husbandry College-Southwest University,Medicinal Plants Joint Research and Development Center,Nyingchi,Tibet 860000;Food Science College,Tibet Agriculture and Animal Husbandry University,Nyingchi,Tibet 860000)

机构地区：[1]西藏农牧学院高原生态研究所,西藏林芝860000 [2]西藏农牧学院-西南大学药用植物联合研发中心,西藏林芝860000 [3]西藏农牧学院食品科学学院,西藏林芝860000

出　　处：《安徽农业科学》2020年第9期172-173,共2页Journal of Anhui Agricultural Sciences

基　　金：西藏自治区科学技术厅科学研究一般项目(XZ2017ZRG-22)。

摘　　要：[目的]从西藏林芝县米瑞乡大花黄牡丹根际土壤中分离耐盐青霉菌菌株,并对其分类地位进行鉴定。[方法]采用高盐察氏培养基从大花黄牡丹根际土壤中分离青霉菌菌株,通过形态学观察和rDNA转录间隔序列ITS测序的方法进行鉴定。[结果]ZP-01菌株菌落无特定形态,镜下可见扫帚状结构,属于青霉菌(MF942929.1),同源性达99%。[结论]耐盐青霉菌ZP-01在濒危植物大花黄牡丹根际土壤微生物群落组成结构研究中具有重要意义。[Objective]To isolate Penicillium strain from the rhizosphere soil of Paeonia ludlowii in Mirui Township,Tibet,and identify its taxonomic status.[Method]The high salt Czapek’s medium were used to isolate Penicillium strain from the rhizosphere soil of Paeonia ludlowii and identified by morphology and ITS sequencing.[Result]ZP-01 formed membranous colony,the typical broom shaped penicillus was found under the microscope,after ITS sequencing,the homology of the Penicillium(MF942929.1)was 99%.[Conclusion]Salt-tolerant Penicillium ZP-01 plays an important role in the study of soil microbial community structure in the rhizosphere of endangered plant Paeonia ludlowii.

关 键 词：根际土壤 青霉菌 分离 鉴定 

分 类 号：S154[农业科学—土壤学]