转氨基作用与氨基酸纸层析实验的改进  

The Improvements of Transamination and Paper Chromatography of Amimo Acid

作　　者：游云悠 魏友煜 左越 黎威巍 罗丽丹 金科华[1,2] YOU Yun-you;WEI You-yu;JIN Ke-hua(Institute of Medicine,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医药研究院,湖北咸宁437100 [2]湖北科技学院基础医学院

出　　处：《湖北科技学院学报（医学版）》2020年第2期99-101,F0002,共4页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北省教育厅科技计划项目(B20160191);湖北科技学院博士启动项目(201820XB015);湖北科技学院教学改革项目(2017XB010)。

摘　　要：目的改进转氨基作用与氨基酸纸层析方式和方法,提高实验效率。方法在新的转氨基反应容器和点样方式下,比较缓冲液类型、转氨基时间、肝匀浆浓度及保存时间、磷酸吡哆醛用量对转氨基的影响。结果适当缩短反应时间、适当稀释和保存肝匀浆对转氨基影响较小;缓冲液类型和磷酸吡哆醛不影响转氨基。结论本研究改进了转氨基反应条件和点样方式,提高了实验效率,改善了实验结果。Objective To improve the pattern and method of transamination and paper chromatography for high experiment efficiency. Methods Compare the effects of substrate’s pH,type of buffer,time of reaction,concentration and preservation time of liver homogenate and the amount of pyridoxal phosphate on transamination under new reaction container and sampling way.Results Shortening the time of reaction,diluting and storing liver homogenate moderately had slight impact on transamination,but the type of buffer and pyridoxal phosphate had no effect on transamination.Conclusion The conditions of transamination and sampling way are improved,and the experiment efficiency as well as results are perfected greatly.

关 键 词：转氨基 氨基酸纸层析 肝匀浆 

分 类 号：R34[医药卫生—基础医学]