茶枝柑皮多糖对H2O2诱导人神经细胞U251的保护作用  

Protective Effect of Tea Branch Orange Peel Polysaccharide on Human Neural Cell U251 Induced by H2O2

作　　者：敬小莉[1] JING Xiao-li(Dazhou Vocational and Technical College,Department of Medical Image Inspection,Dazhou 635000,China)

机构地区：[1]达州职业技术学院医学影像检验系,四川达州635000

出　　处：《现代食品科技》2020年第4期39-45,共7页Modern Food Science and Technology

基　　金：四川省科技扶贫项目(2018NFP0112)。

摘　　要：本论文研究了茶枝柑皮多糖H2O2诱导人神经细胞U251的保护作用。采用酸提法、经纯化后得到茶枝柑皮多糖。建立人神经细胞U251细胞损伤模型,测定细胞增殖率,采用0.30 mg/mL、0.60 mg/mL、1.25 mg/mL、2.50 mg/mL茶枝柑皮多糖干预,计算细胞存活率,检测超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)的含量水平。茶枝柑皮多糖CPA-Ⅰ相对分子质量为7.96×10^4,20、50、180、375μmol/L H2O2细胞增殖率分别为91.60%、84.40%、60.00%、37.40%、12.30%与对照组比较,具有统计学差异(p<0.05)。180.0μmol/L H2O2导致人神经细胞U251损伤的最佳浓度。0.30、0.60、1.25、2.50mg/mL茶枝柑皮多糖细胞存活率分别为64.30%、76.10%、93.40%、98.20%与模型组53.00%比较,具有统计学差异(p<0.05)。2.50mg/mL茶枝柑皮多糖对细胞损伤模型干预的效果最明显,抗氧化作用最强。茶枝柑皮多糖对H2O2诱导人神经细胞U251具有一定的保护作用,通过调控SOD、GSH-Px、GSH、MDA水平起到抗氧化作用。In this work, the protective effect of tea branch orange peel polysaccharide H2O2 on human neural cell U251 was investigated. The polysaccharide was extracted by acid method and further purified. To The injury model of human neural cell U251 was established to detect the cell proliferation rate. The cell survival rate was calculated and the content of superoxide dismutase(SOD), malondialdehyde(MDA), glutathione peroxidase(GSH-PX) and glutathione(GSH) were measured by the intervention of 0.30 mg/mL, 0.60 mg/mL, 1.25 mg/mL and 2.50 mg/m L tea branch orange peel polysaccharide. The relative molecular weight of cpa-i was 7.96×10^4. The proliferation rate of 20, 50, 180 and 375 μmol/L H2O2 cells were 91.60%, 84.40%, 60.00%, 37.40% and 12.30% respectively, which was statistically different from that of the control group(p<0.05). The optimal concentration of H2O2(180.0 μmol/L) for the injury of U251 cells. The cell survival rates of 0.30, 0.60, 1.25 and 2.50 mg/mL tea branch orange peel polysaccharide cells were 64.30%, 76.10%, 93.40% and 98.20%, respectively, which were higher than that of the model group(53.00%, p<0.05). The 2.50 mg/ml tea branch orange peel polysaccharide had the most obvious effect on cell injury model, and the strongest antioxidant effect. Tea branch orange peel polysaccharide had a certain protective effect on human neural cell U251 induced by H2O2, and played an antioxidant role by regulating the levels of SOD, GSH Px, GSH and MDA.

关 键 词：茶枝柑皮多糖 分离 纯化 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学]