鳜pep和gh基因SNP标记与驯食性状的关联分析  

Single nucleotide polymorphisms in pepsinogen gene, growth hormone gene and their association with food habit domestication traits in Siniperca chuatsi

作　　者：窦亚琪[1,2] 梁旭方 高俊杰[1] 何珊 石林杰[1] 史登勇 DOU Yaqi;LIANG Xufang;GAO Junjie;HE Shan;SHI Linjie;SHI Dengyong(College of Fisheries,Chinese Perch Research Center,Huazhong Agricultural University/Innovation Base for Chinese Perch Breeding,Key Lab of Freshwater Animal Breeding,Ministry of Agriculture and Rural Affairs,Wuhan 430070,China;Hubei Aquaculture Technology Extension Center(Hubei Aquatic Breeds Introduction and Breeding Center),Wuhan 430070,China;Hubei Province Aquatic Stains Testing Station,Wuhan 430070,China)

机构地区：[1]华中农业大学水产学院,华中农业大学鳜鱼研究中心,农业农村部鳜鱼育种创新基地,农业农村部淡水生物繁育重点实验室,湖北武汉430070 [2]湖北省水产技术推广总站(湖北省水产良种引进繁育中心),湖北武汉430070 [3]湖北省水产良种试验站,湖北武汉430070

出　　处：《中国水产科学》2020年第5期485-493,共9页Journal of Fishery Sciences of China

基　　金：农业农村部现代农业产业技术体系专项资金资助项目(CARS-46);国家重点研发计划“蓝色粮仓科技创新”项目(2018YFD0900400);武汉市青年科技晨光计划项目(2017050304010318).

摘　　要：以鳜(Siniperca chuatsi)选育群体为实验材料,在易驯食与不易驯食鳜转录组Unigene数据库中共预测到4809个SNP位点,其中胃蛋白酶基因(pepsinoge, pep)和生长激素基因(growth hormone, gh)均为转录组筛选获得的鳜驯食性状候选基因,本研究将候选基因上的多态SNP位点在易驯食和不易驯食鳜群体中进行基因分型,并与鳜驯食性状进行关联分析。在易驯食与不易驯食的鳜群体中共发现5个单核苷酸(SNP)多态性位点,有效等位基因(N_e)在1.1959~1.7001,观测杂合度(H_o)和期望杂合度(H_e)分别分布于0.1800~0.3585和0.1655~0.4160,多态信息含量(PIC)为0.2477,全部位点都属于中度多态性位点。结果表明SNP位点pep-A T/C中2种基因型TT和CT与鳜驯食性状呈高水平显著相关(P<0.05),组合得到的3种基因型Genotype1(CT,CC/CT/TT,AA,AA,TT)、Genotype2(TT,CC/CT/TT, AA, AA, TT)和Genotype3(TT, CC, AA, AA, TT)也与鳜驯食性状呈高水平显著相关(P<0.05),显著影响鳜驯食性状表型,其中Genotype2相关性最高,可作为最优基因型个体进行选育。本研究在鳜pep和gh基因中鉴定出与驯食性状呈显著关联的SNP分子标记,为加快易驯食鳜新品种的基因辅助选育提供有效的SNP分子标记。Mandarin fish is one of the most commercially important freshwater fish in Chinese aquaculture. Once the fry of mandarin fish start feeding, in the wild they exclusively eat live prey fish;therefore in cultivation they refuse dead prey fish or artificial diets, contributing to the challenges of breeding this fish. Currently, the source of live feed for the intensively cultured mandarin fish is also cultivated. The challenges in cultivating live feed for mandarin fish include extremely high cultivation costs, difficulties in maintaining stock supply all year round, and disease prevention, which is very difficult in the cultivation process. Therefore introducing artificial diets in domesticated mandarin fish could resolve these challenges. Based on the existing research, this study analyzed the transcriptome data of mandarin fish, and the differences in expression of pepsinogen(pep) gene and growth hormone(gh) gene were detected. Genes pep and gh are considered as candidate genes for food habit traits in domesticated mandarin fish. In this study, single nucleotide polymorphisms(SNPs) on candidate genes were sequenced and typed in a feeder group fed dead prey fish diets and a non-feeder control group, and evaluated for associations with food habit domestication traits in 103 mandarin fish. The feeder means the fish accepts dead prey fish, and the non-feeder means the fish refuses to eat dead prey fish. In total, five SNPs were identified in pep and gh genes. The effective allele numbers ranged from 1.1959 to 1.7001, with a mean of 1.4274 alleles per locus. The observed(H_o) and expected heterozygosities(H_e) values ranged from 0.1800 to 0.3585, and from 0.1655 to 0.4160, respectively. The value of polymorphism information content(PIC) was 0.2477, which is a classification of the median polymorphism level in the mandarin fish population. These results showed that the two genotypes TT and CT in the pep-A T/C of the SNP locus were significantly correlated with the high level of the food habit domestication traits in ma

关 键 词：翘嘴鳜 驯食性状 单核苷酸多态性(SNP) 关联分析 

分 类 号：S963[农业科学—水产养殖]