蜂蜇草萃取物对胡蜂蜂毒致人红细胞溶血的拮抗作用  

Antagonistic effect of the Fengzhecao extract against human red blood cell hemolysis induced by wasp venom

作　　者：蒲运刚 田仁富 吴钢 夏燕 王业超 黄承 朱顺 黄长江 杨希 Pu Yungang;Tian Renfu;Wu Gang;Xia Yan;Wang Yechao;Huang Cheng;Zhu Shun;Huang Changjiang;Yang Xi(Department of Critical Care Medicine,the Affiliated Central Hospital of Enshi Autonomous Prefecture of Hubei Minzu University,Enshi 445000,Hubei,China;Department of Blood Transfusion,the Affiliated Central Hospital of Enshi Autonomous Prefecture of Hubei Minzu University,Enshi 445000,Hubei,China)

机构地区：[1]湖北民族大学附属恩施土家族苗族自治州中心医院重症医学科,湖北恩施445000 [2]湖北民族大学附属恩施土家族苗族自治州中心医院输血科,湖北恩施445000 [3]湖北民族大学风湿性疾病发生与干预湖北省重点实验室,湖北恩施445000

出　　处：《中华危重病急救医学》2020年第2期226-229,共4页Chinese Critical Care Medicine

基　　金：湖北省临床输血重点专科建设项目(2019-4)。

摘　　要：目的:研究蜂蜇草萃取物对胡蜂蜂毒致人红细胞溶血的拮抗作用。方法:采用水提取法对干蜂蜇草进行萃取,真空干燥获得蜂蜇草萃取物,配制成1 g/L备用;从胡蜂工蜂尾部收集蜂毒原液备用。获取A、B、O、AB型健康献血者悬浮红细胞液制成洗涤红细胞,调节红细胞计数(4.0~80.0)×10^9/L(血细胞计数板上计数红细胞数量1~20个/小格),按处理因素分成生理盐水对照组(NS组,红细胞液200μL+生理盐水20μL)、蜂蜇草萃取物组(FZC组,红细胞液200μL+蜂蜇草萃取物10μL+生理盐水10μL)、蜂毒组(FD组,红细胞液200μL+蜂毒10μL+生理盐水10μL)、蜂蜇草萃取物+蜂毒组(FCD组,红细胞液200μL+蜂蜇草萃取物10μL+蜂毒10μL),每组10份。将各组溶液置于玻璃试管中,放入37℃温箱,10 min后于显微镜下直接观察血细胞计数板并进行红细胞计数,比较不同血型相同处理因素之间以及相同血型不同处理因素组之间红细胞计数的差异。结果:在相同处理因素下,各血型间红细胞计数差异无统计学意义,NS组中A、B、O、AB型红细胞计数(×10^9/L)分别为5.567±1.368、5.146±1.690、4.577±0.774、5.197±1.587(F=0.852,P=0.475),FZC组分别为5.751±1.489、5.268±1.418、4.727±1.174、5.298±1.229(F=0.987,P=0.410),FD组分别为0.546±0.450、0.804±0.428、0.679±0.283、0.846±0.453(F=1.089,P=0.366),FCD组分别为5.532±1.330、5.051±1.596、4.589±0.879、5.140±1.492(F=0.820,P=0.492)。相同血型不同处理因素组间比较红细胞计数:在FZC组与NS组间比较差异无统计学意义,说明蜂蜇草萃取物对红细胞溶血无影响;而在FD组则明显低于NS组(均P<0.05),说明蜂毒对红细胞有明显的溶血作用;但在FCD组与NS组间比较红细胞计数差异也无统计学意义,说明蜂蜇草萃取物拮抗蜂毒的溶血作用而不影响红细胞计数;而FCD组红细胞计数明显高于FD组(均P<0.05),进一步说明蜂蜇草萃取物拮抗蜂毒的溶血作用。结论:胡蜂�Objective To explore the antagonistic effect of Fengzhecao extract against human red blood cell(RBC)hemolysis induced by wasp venom.Methods Water extract method was used to extract dried Fengzhecao and vacuum-dried to obtain Fengzhecao extract.It was diluted into 1 g/L for next use.Wasp venom was collected from the wasp workers.A,B,O,AB type healthy blood donors'suspended RBC solution was obtain to make washed RBC solutions and adjust the RBCs count(4.0-80.0)×10^9/L(the number of RBC counted on the hemocytometer is 1-20 cells/small checker).According to treatment factors,they were divided into the normal saline controlled group(NS group;200μL RBC solution+20μL normal saline),Fengzhecao extract group(FZC group;200μL RBC solution+10μL Fengzhecao extract+10μL normal saline),wasp venom group(FD group;200μL RBC solution+10μL wasp venom+10μL normal saline),and Fengzhecao extract+wasp venom group(FCD group;200μL RBC solution+10μL Fengzhecao extract+10μL wasp venom),with 10 blood samples per group of every blood type.The solutions were put into the glass test tube respectively,and then into 37℃water bath thermostat.After 10 minutes,the blood cell counting plate was directly observed under the microscope and the RBCs was counted.Differences in RBC count was compared between the same treatment factors of different blood types and between different treatment factor groups of the same blood type.Results There was no statistically significant difference in RBC count between blood types under the same treatment factors.The RBC count(×10^9/L)of the type A,B,O,AB in the NS group were 5.567±1.368,5.146±1.690,4.577±0.774,5.197±1.587(F=0.852,P=0.475),the FZC group were 5.751±1.489,5.268±1.418,4.727±1.174,5.298±1.229(F=0.987,P=0.410),the FD group were 0.546±0.450,0.804±0.428,0.679±0.283,0.846±0.453(F=1.089,P=0.366),and the FCD group were 5.532±1.330,5.051±1.596,4.589±0.879,5.140±1.492(F=0.820,P=0.492),respectively.Comparison of RBC count between groups with different treatment factors of the same blood

关 键 词：蜂 咬伤 蜇伤 蜂蜇草 蜂毒 血型 溶血 

分 类 号：R457.1[医药卫生—治疗学]