花花柴抗逆相关转录因子WRKY的鉴定及分析  

Identification and Analysis of the Stress-related WRKY Transcription Factor in Karelinia Caspica

作　　者：余从巧 邓芳[1] 赵红山 徐靖辰 王彦芹[1,2] Yu Congqiao;Deng Fang;Zhao Hongshan;Xu Jingchen;Wang Yanqin(College of Life Sciences,Tarim University,Alar,Xinjiang 843300;Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Xinjiang Production&Construction Corps.Alar,Xinjiang 843300)

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300

出　　处：《塔里木大学学报》2020年第1期28-35,共8页Journal of Tarim University

基　　金：塔里木大学大学生创业创新项目“利用VIGS技术分析花花柴WRKY基因的功能”(107572017002);国家自然科学基金“沙漠植物花花柴花器官耐高温的生物学功能分析”(31660085)。

摘　　要：WRKY转录因子作为参与生物或非生物胁迫应答的重要基因家族,在植物逆境胁迫的响应过程中起着重要作用。花花柴作为沙漠植物,具有极强的广谱抗逆性,发掘并应用花花柴的抗逆基因资源对研究植物逆境生物学及抗逆性分子育种具有重要意义。本研究通过对沙漠植物花花柴的转录组学分析,筛选出与高温胁迫相关的15个差异表达的WRKY基因。并将这15个基因与拟南芥、水稻的WRKY基因构建系统发育树,通过对同亚族同源性较高的基因功能及其顺式作用元件的功能预测,推测花花柴WRKY基因可能参与了极端温度、干旱、高盐、病原菌等逆境胁迫响应,并对与高温胁迫响应相关的2个花花柴WRKY基因进行表达模式分析。该结果将为荒漠植物及其基因资源的发掘利用提供一定的参考价值。The WRKY transcription factor family,as an important gene family involved in the response to biological or abiotic stress,plays an important role in the response to stress in plants.Karelinia caspica,a desert plant,was wide and strong resistance to stress.Exploring and applying the stress tolerance genes of Karelinia caspica,which was of great significance to the study of plant stress biolo gy and stress resistance molecular breeding.Fifteen differentially expressed WRKY genes related to high temperature stress were screened by transcriptome analysis of Karelinia caspica.15 genes were combined with the WRKY genes from Arabidopsis thaliana and Rice to construct phylogenetic trees.By predicting the functions of genes with higher homology of the same subgroup and their cis-act ing elements,it is speculated that the WRKY gene of Kareliniacaspicamay be involved in the response to stress such as temperature,drought,high salt and pathogenic bacteria.The expression patterns of 2 WRKY genes related to temperature stress response were ana lyzed.The results will provide some reference value for the exploration and utilization of desert plants and their genetic resources.

关 键 词：沙漠植物 花花柴 WRKY转录因子 系统发育分析 表达模式分析 

分 类 号：Q71[生物学—分子生物学]