燕麦籽粒皮裸性相关AP2/ERF转录因子基因的克隆  

Cloning of AP2/ERF Transcription Factor Gene Related to Oat Kernel Covering

作　　者：张鸣凡 陈志伟 贾举庆[1] 张晓军[2] 李欣[2] 张春来[1] 张美俊[1] 杨武德[1] ZHANG Mingfan;CHEN Zhiwei;JIA Juqing;ZHANG Xiaojun;LI Xin;ZHANG Chunlai;ZHANG Meijun;YANG Wude(College of Agronomy,Shanxi Agricultural University,Taigu 030801,China;Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences,Key Laboratory of Crop Genetics and Molecular Improvement,Shanxi Province,Key Laboratory of Crop Genetic Resources and Germplasm Creation,Loess Plateau,Ministry of Agriculture,Taiyuan 030031,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]山西省农业科学院作物科学研究所,作物遗传与分子改良山西省重点实验室,农业部黄土高原作物基因资源与种质创制重点实验室,山西太原030031

出　　处：《山西农业科学》2020年第5期658-663,共6页Journal of Shanxi Agricultural Sciences

基　　金：山西省重点研发计划一般项目(201903D221073);山西省重点研发计划重点项目(201703D211001-03-01);山西农谷建设科研专项(SXNGJSKYZX201702)。

摘　　要：燕麦籽粒皮裸性是燕麦籽粒带壳与籽粒不带壳(裸粒)的一对相对性状,反映了籽粒脱离谷壳的难易程度,研究该性状对作物育种、起源驯化具有重要作用。以二倍体皮、裸燕麦为材料,采用电子克隆的方法,克隆到2个AP2/ERF转录因子基因AsNud1和AsNud2,对这2个基因的结构分析发现,AsNud1和AsNud2为旁系同源基因;二者与HvNud都具有很高的相似性,其中,AsNud2与HvNud的相似性更高;皮燕麦的AsNud1和AsNud2编码的氨基酸有提前终止现象,而裸燕麦中的这2个基因则没有此现象。对AsNud1和AsNud2表达特性分析发现,AsNud1和AsNud2在皮、裸燕麦的花器官中都有表达,但表达模式不同,其中,AsNud1在CIav2921中的表达量低于在CIav9008中的表达量,但二者差异没有达到显著水平;而AsNud2在CIav2921中的表达量显著高于在CIav9008中的表达量。因此推测,AsNud可能参与调控燕麦籽粒麸皮的发育。Oat kernel covering refers to whether the husk wrapped outside the kernel is easy to separate from the kernel after ripening.It is important to study this trait for crop breeding and origin domestication.Two AP2/ERF transcription factor genes,AsNud1 and AsNud2,were cloned from diploid oat by electronic cloning.The structural analysis of the two genes showed that AsNud1 and AsNud2 were homologous genes,these two genes had high similarity with HvNud,among which AsNud2 and HvNud had higher similarity.The amino acids encoded by AsNud1 and AsNud2 in husked oat had early termination,but the two genes in naked oat did not.The expression analysis of AsNud1 and AsNud2 showed that both AsNud1 and AsNud2 were expressed in the flower organs of oats,but the expression patterns were different,the expression of AsNud1 in CIav2921 was lower than that in CIav9008,but the difference was not significant,the expression of AsNud2 in CIav2921 was significantly higher than that in CIav9008.Therefore,it is suggested that gene AsNud may be involved in the regulation of oat kernel development.

关 键 词：燕麦 AP2/ERF转录因子 AsNud 籽粒皮裸性 

分 类 号：S512.6[农业科学—作物学]