基于网络药理学的柴胡-白芍药对抗抑郁作用机制研究  

Study on the Mechanism of Anti-Depression Effect of Chaihu (Radix Bupleuri)-Baishao (Radix Paeoniae Alba) Drug Pair based on Network Pharmacology

作　　者：常泽 韩振蕴[2] 曹云松[2] 刚丽丽 田丹枫 高强[1] 张丹丹[1] 林景峰 王育纯 刘甘露 CHANG Ze;HAN Zhenyun;CAO Yunsong;GANG Lili;TIAN Danfeng;GAO Qiang;ZHANG Dandan;LIN Jingfeng;WANG Yuchun;LIU Ganlu(Beijing University of Chinese Medicine,Beijing,100029;Dongfang Hospital,Beijing University of Chinese Medicine;Changping Hospital of Integrated Traditional Chinese and Western Medicine,Beijing)

机构地区：[1]北京中医药大学,北京市100029 [2]北京中医药大学东方医院,北京市100029 [3]北京市昌平中西医结合医院

出　　处：《中医杂志》2020年第8期700-705,共6页Journal of Traditional Chinese Medicine

基　　金：国家食品药品监督管理总局委托研究课题。

摘　　要：目的基于网络药理学探讨柴胡白芍药对抗抑郁的可能作用机制。方法通过检索TCMSP和TCMID数据库筛选出柴胡白芍药对的潜在活性成分及靶点,采用GeneCard与OMIM数据库获得抑郁症的作用靶点,得到药物与疾病的交集靶点,并运用Cytoscape 3.7.1软件绘制活性成分靶点疾病交互网络图。采用String数据库构建靶点相互作用PPI网络,通过Cytoscape 3.7.1的Network Analyzer工具进行交集靶点的拓扑属性分析,运用其插件Cluster Maker进行模块化分析,并对交集靶点进行基因GO功能分析和KEGG通路富集分析。结果共筛选出柴胡白芍的潜在活性成分14个及相应靶点47个,抑郁症相关靶点7678个,交集靶点45个。PPI网络模块化分析结果显示,45个靶点主要分为3个模块,模块一靶点主要参与对雌二醇的反应、对钴离子的反应、神经元凋亡过程的正调控、无配体的外源性凋亡信号通路及RNA聚合酶Ⅱ启动子的转录起始等生物学过程。模块二靶点主要参与腺苷酸环化酶抑制G蛋白偶联乙酰胆碱受体信号、磷脂酶C激活G蛋白偶联乙酰胆碱受体信号蛋白、胆碱能突触传递和G蛋白偶联乙酰胆碱受体信号通路等生物过程发挥抗抑郁作用。KEGG通路分析主要参与了多种疾病通路、突触通路及代谢通路等。结论柴胡白芍药对抗抑郁作用具有多成分、多靶点、多通路的作用机制特点,其中ACHE、MAOA、SLC6A4、MAOB重要靶点已被证明与抑郁症的治疗密切相关。Objective To explore the possible mechanism of Chaihu(Radix Bupleuri)-Baishao(Radix Paeoniae Alba) drug pair against depression based on network pharmacology. Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and Traditional Chinese Medicine Integrated Database(TCMID) databases were used to screen out the potential active components and targets of the drug pair. The GeneCard and OMIM databases were used to obtain targets for depression, and the target of drug and disease was obtained. Cytoscape 3.7.1 software was used to draw an interactive network map of the active ingredient target disease. The protein-protein interaction(PPI) network was constructed using the String database. The topo-logy property of the intersection target was analyzed by the Network Analyzer tool of Cytoscape 3.7.1. The plug-in Cluster Maker was used for modular analysis, and the GO target function analysis and KEGG pathway enrichment analysis were performed on the intersection target. Results A total of 14 potential active components, 47 corresponding targets, 7678 depression-related targets, and 45 overlapping target sites were screened in the drug pair. The PPI network modular analysis showed that 45 targets were mainly divided into 3 modules. The module 1 target mainly participated in the biological processes such as reaction to estradiol, the reaction to cobalt ions, the positive regulation of neuronal apoptosis, ligand-free exogenous apoptosis signaling pathways and transcription initiation of the RNA polymerase Ⅱ promoter. Module 2 targets are primarily involved in the biological processes such as adenylate cyclase inhibition of G protein-coupled acetylcholine receptor signaling, phospholipase C activation of G-protein coupled acetylcholine receptor signaling proteins, cholinergic synaptic transmission, and G-protein coupled acetylcholine receptors signaling pathways, exerting antidepressant effects. KEGG pathway analysis is mainly involved in a variety of disease pathways, synaptic

关 键 词：柴胡 白芍 抑郁症 药对 网络药理学 

分 类 号：R285[医药卫生—中药学]