NGF通过调节Sirt1蛋白对足细胞凋亡的保护作用  

NGF protects podocyte apoptosis by regulating Sirt1 expression

作　　者：鄂静[1,2] 马丹娜 曹丽[1,2] 李博[1,2] 张秀梅[1,2] 郑亚莉[1,2] E Jing;MA Danna;CAO Li;LI BO;ZHANG Xiumei;ZHENG Yali(Department of Nephropathy,People's Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China;First Affiliated Hospital of Northwest University for Nationalities,Yinchuan 750002,China)

机构地区：[1]宁夏回族自治区人民医院肾脏内科,宁夏银川750002 [2]西北民族大学第一附属医院,宁夏银川750002

出　　处：《宁夏医学杂志》2020年第4期294-296,共3页Ningxia Medical Journal

基　　金：国家自然科学基金项目(81060066,81860136);西北民族大学中央高校项目(31920180091,31920170184);宁夏回族自治区自然科学基金项目(NZ17186);宁夏回族自治区重点研发计划重大重点项目(东西部项目2018BFG02010)。

摘　　要：目的探讨神经生长因子(NGF)通过调节Sirt1蛋白表达对足细胞凋亡保护的作用机制,为临床治疗提供新的生物靶点。方法培养离体分化成熟的肾小球足细胞,转染空白RNA(con siRNA)或siNGF质粒,通过免疫印迹法测定Sirt1和凋亡表达蛋白Cleaved Caspase3的蛋白量,并观察给予不同浓度NGF刺激后上述蛋白的变化水平,观察NGF与Sirt1的相互关系及对足细胞的作用。结果分化良好的足细胞给予不同浓度的NGF刺激后,可见随着NGF浓度增加组蛋白去乙酰酶Sirt1的蛋白表达也增加;使用ViaFectTM转染试剂盒给足细胞转染(con siRNA)和siNGF,通过免疫印迹验证沉默NGF的表达,结果显示在siNGF沉默NGF表达组Sirt1蛋白表达降低,足细胞凋亡发生增多。而同时对siNGF组的细胞给予NGF刺激,与siNGF组比较,Cleaved Caspase3的蛋白表达减少,足细胞凋亡发生缓解。结论NGF通过增加Sirt1的表达对足细胞发挥保护作用,NGF/SIRT1轴可能成为一个新的干预足细胞损害的治疗靶点。Objective To explore the protective mechanism of NGF on podocyte apoptosis by regulating Sirt1 expression and to provid a new biological target for clinical treatment.Methods The immortalized mouse podocyte was cultured in vitro and were transfected with control siRNA or siNGF vector.The protein level of Sirt1 and apoptosis associated protein cleaved-caspase 3 were measured by Western blotting.In addition,podoctyes were also given different concentration of NGF to detect the expression of Sirt1 and cleaved-caspase 3 in order to find the relationship of NGF and Sirt1.Results After simulated by NGF,the expression of Sirt1 was increased with the different concentration of NGF in podocyte.The protein expression of SIRT1 decreased and podocyte apoptosis increased in the NGF silenced group.Compared with the siNGF group,the protein expression of cleared-caspase 3 decreased,and the apoptosis of podocytes was alleviated.Conclusion NGF can plays a key role in protection podocyte by regulating the expression of Sirt1,NGF/Sirt1 may be a new biological target for preventing podocyte injury.

关 键 词：神经生长因子 Sirt1蛋白 足细胞 

分 类 号：R692.6[医药卫生—泌尿科学]