亚洲玉米螟幼虫利用反吐液抑制玉米叶片丁布合成的研究  

Study on Ostrinia furnacalis larvae inhibit DIMBOA biosynthesis in maize leaves using regurgitant

作　　者：高超男 宋鹏飞[1] 姚双艳 盛子耀 李为争[1] 罗梅浩[1] 原国辉[1] GAO Chaonan;SONG Pengfei;YAO Shuangyan;SHENG Ziyao;LI Weizheng;LUO Meihao;YUAN Guohui(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学植物保护学院,河南郑州450002

出　　处：《河南农业大学学报》2020年第2期248-253,275,共7页Journal of Henan Agricultural University

基　　金：国家农业部粮食丰产增效重点专项(2018YFD0300706)。

摘　　要：以玉米品种“浚单20”为材料,设置了机械损伤组、亚洲玉米螟幼虫取食组和模拟取食组3个外源诱导因素,每个因素内部又设置了不同损伤强度和诱导时间,用高效液相色谱-质谱联用仪系统测定不同处理组玉米叶片丁布含量变化。结果发现诱导因素类型对丁布含量调控起着主导作用,诱导时间和损伤强度从属前者发挥作用。同时发现,对照组玉米叶片中丁布的平均含量为(419.55±30.53)μg·g^-1,仅机械损伤能刺激丁布合成(492.39±53.00)μg·g^-1,而亚洲玉米螟取食能显著抑制丁布的合成(439.26±32.76)μg·g^-1。将幼虫的反吐液涂布在机械损伤口处理后的叶片,能够再现亚洲玉米螟取食对丁布合成的抑制作用(451.39±29.22)μg·g^-1,说明亚洲玉米螟主要是通过反吐液抑制丁布合成的。本研究表明,丁布并不是昆虫取食诱导性防卫代谢物。We exposed the test material maize(var.Xundan 20)plants to three types of exogenous induction factors,i.e.mechanical damage(punching holes on the leaves),feeding by larvae of Ostrinia furnacalis(Guenée),and simulated feeding(larval regurgitant of O.furnacalis applied on punched holes),and designed different levels of damage strength and induction time within each group.After induction,the DIMBOA content changes were measured using high-performance liquid chromatography coupled with mass spectrometry(HPLC-MS).The results showed that the type of exogenous induction factors played a dominant role in DIMBOA biosynthesis,while induction time and damage strength subordinated to the former.Meanwhile,we found that the mean DIMBOA content in undamaged maize leaves was(419.55±30.53)μg·g^-1.Among the three exogenous induction factors,only mechanical damage could stimulate DIMBOA biosynthesis(492.39±53.00)μg·g^-1 in contrast,feeding by O.furnacalis larvae could significantly inhibit DIMBOA biosynthesis(439.26±32.76)μg·g^-1.Coating mechanical wound with larval regurgitant could reproduce the inhibition effect of true larval feeding(451.39±29.22)μg·g^-1,suggesting that O.furnacalis larvae could inhibit DIMBOA biosynthesis via regurgitant during feeding.This study indicates that DIMBOA is not an insect feeding-induced metabolite for defense.

关 键 词：亚洲玉米螟 玉米 丁布 外源诱导 反吐液 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治] S433.1[农业科学—植物保护]