利用SSR技术快速鉴定2个辣椒杂交品种纯度  

Rapid Identification of Two Pepper Hybrid Cultivars Purity by SSR Markers

作　　者：陈琛[1] 罗俊杰[1] 陈卫国 CHEN Chen;LUO Junjie;CHEN Weiguo(Institute Biotechnology,Gansu Academy of Agricultural Science,Lanzhou Gansu 730070,China;Gansu Lüxing Agricultural Technology Co.,Ltd.,Lanzhou Gansu 730070,China)

机构地区：[1]甘肃省农业科学院生物技术研究所,甘肃兰州730070 [2]甘肃绿星农业科技有限责任公司,甘肃兰州730070

出　　处：《甘肃农业科技》2020年第4期53-58,共6页Gansu Agricultural Science and Technology

基　　金：甘肃省农业科学院科技支撑计划项目(2017GAAS50)。

摘　　要：利用SSR分子标记技术,对辣椒杂交种甘科4号和甘科10号及其父母本进行引物筛选和纯度鉴定,旨在建立供试品种的高效纯度鉴定体系,并对公布的辣椒SSR核心引物进行验证。结果表明,2个品种各筛选出1对引物在亲本间有明显差异,在杂交种中表现为共显性,分别为Es330和Epms923。利用这2对引物分别对供试品种进行纯度鉴定,甘科4号的纯度为96.4%,与田间种植鉴定纯度98.2%相差1.8百分点;甘科10号的纯度为91.8%,与田间种植鉴定纯度92.7%相差0.9百分点。说明筛选出的2对引物对甘科4号和甘科10号种子纯度的鉴定结果真实可靠,可以用于杂交种纯度的高效快速鉴定。In this study,pepper hybrid cultivars Ganke 4、Ganke 10 and their parental inbred lines were used to identify hybrid purity by SSR markers,aiming at establishing a quick and efficient purity identification system for tested varieties,verifying the published pepper SSR core primers at the same time.The results showed that there was one primer pair selected in each cultivars,named Es330 and Epms923,which had significant difference between the parental inbred lines,and showed co-dominance in the hybrids.Using the two primer pairs to identify the purity of the tested varieties,the purity of Ganke 4 was 96.4%,which had 1.8 percentage point difference from the purity of 98.2%identified in field.the purity of Ganke 10 was 91.8%,which had 0.9 percentage point difference from the purity of 92.7%by field identification.It was proved that the identification of hybrid purity of Ganke 4 and Ganke 10 were authentic and reliable,the two SSR markers could be used for efficient and rapid identification of hybrid purity.

关 键 词：辣椒 SSR分子标记 纯度鉴定 

分 类 号：S641.3[农业科学—蔬菜学]