丽江乌头中二萜生物碱成分  

Diterpenoid alkaloids from Aconitum forrestii Stapf

作　　者：尚语杉 刘为 任佳俐 黄帅[1] 周先礼[1] 高峰[1] SHANG Yu-shan;LIU Wei;REN Jia-li;HUANG Shuai;ZHOU Xian-li;GAO Feng(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China)

机构地区：[1]西南交通大学生命科学与工程学院,成都610031

出　　处：《天然产物研究与开发》2020年第3期435-440,共6页Natural Product Research and Development

基　　金：国家自然科学基金(31870329)。

摘　　要：本研究采用硅胶柱层析对丽江乌头(Aconitum forrestii Stapf)中的二萜生物碱成分进行分离,并利用NMR、MS以及与文献对比的方法和手段,对分离得到的14个化合物进行结构鉴定,确定结构分别为sachaconitine(1)、8-O-methylsachaconitine(2)、14-O-acetylsachaconitine(3)、6-epiforsticine(4)、8-O-methyltalatisamine(5)、14-methyltalatisamine(6)、austroconitine B(7)、hemsleyadine(8)、vilmorrianine A(9)、yunaconitine(10)、N-deethyl-N-19-didehydrosachaconitine(11)、14-debenzoylfranchetine(12)、deacetylheterophylloidine(13)和sczukinine(14),其中化合物1~9,11~14均为首次从该植物中分离得到。采用MTT法考察了部分化合物对阿霉素诱导的H9c2心肌细胞损伤的保护活性,结果显示,当浓度为40μM时,化合物5的细胞存活率为65.25%(阿霉素的细胞存活率为60.24%),表现出一定的心肌细胞保护活性,其他化合物均未表现出活性。Fourteen diterpenoid alkaloids,including sachaconitine(1),8-O-methylsachaconitine(2),14-O-acetylsachaconitine(3),6-epiforsticine(4),8-O-methyltalatisamine(5),14-methyltalatisamine(6),austroconitine B(7),hemsleyadine(8),vilmorrianine A(9),yunaconitine(10),N-deethyl-N-19-didehydrosachaconitine(11),14-debenzoylfranchetine(12),deacetylheterophylloidine(13),sczukinine(14),were isolated from Aconitum forrestii Stapf by silica gel column chromatography and identified by NMR,MS and literature comparison.Compounds 1-9,11-14 were isolated from this plant for the first time.The protective activity against adriamycin induced H9 c2 rate of compound 5 was 65.25%compared with 60.24%of adriamycin when the concentration was 40μM,which indicated compound 5 behaved certain protective activity,while none of the other compounds showed any activity.

关 键 词：乌头属 丽江乌头 二萜生物碱 心肌细胞保护活性 

分 类 号：R284.1[医药卫生—中药学]