基于UPLC-Q-TOF-MS技术的玫瑰石斛生物碱研究  

Study on alkaloids from stems of Dendrobium crepidatum based on UPLC-Q-TOF-MS

作　　者：李振坚[1] 周文雅 韩彬 王元成 曾艺芸 卢思聪[2] 孙振元[1] LI Zhen-jian;ZHOU Wen-ya;HAN Bin;WANG Yuan-cheng;ZENG Yi-yun;LU Si-cong;SUN Zhen-yuan(Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091,China;Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China)

机构地区：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091 [2]中国科学院植物研究所,北京100093

出　　处：《天然产物研究与开发》2020年第3期482-488,426,共8页Natural Product Research and Development

基　　金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2017MB001);国家林业局948项目(2015-4-10)。

摘　　要：玫瑰石斛(Dendrobium crepidatum)是兰科石斛属多年生附生草本植物,具有较高保健和药用价值。本研究利用超高效液相色谱-四级杆飞行时间质谱(UPLC-Q-TOF-MS)技术,研究了玫瑰石斛茎中生物碱成分和相对含量。从玫瑰石斛茎中共鉴定出15种成分:相对含量大于50μg/g的成分有两种,玫瑰石斛胺174.844μg/g,玫瑰石斛啶碱92.862μg/g;含量介于5~50μg/g间的成分有玫瑰石斛碱B、玫瑰石斛碱D、玫瑰石斛碱、玫瑰石斛碱C、玫瑰啶碱B;含量低于5μg/g的成分有玫瑰石斛素、N-异戊烯基石斛碱、石斛碱、N-异戊烯基石斛星碱、杓唇石斛素、玫瑰石斛碱E、玫瑰酚A、石斛酮碱。该研究首次在玫瑰石斛中发现4种倍半萜类生物碱。玫瑰石斛生物碱以八氢中氮茚类生物碱为主。Dendrobium crepidatum Lindl.ex Paxt.was a perennial epiphytic herb with high health and medicinal value in Dendrobium SW,Orchidaceae.The components and contents of alkaloids were studied by UPLC-Q-TOF-MS from the stems of D.crepidatum.Fifteen alkaloids and bibenzyls components were identified from the stems,in which twelve components were alkaloids and three components were bibenzyls.There were two components with contents more than 50 μg/g,crepidine174.844 μg/g and crepidamine 92.862 μg/g respectively;5-50 μg/g of 15 components:dendrocrepidine B,dendrocrepidine D,dendrocrepine,dendrocrepidine C,and homocrepidine B;crepidatin,N-isopentenyl-dendrobinium,dendrobine,N-isopentenyl-dendroxinium,moscatilin,dendrocrepidine E,crepidatuol A,and nobilonine were found in 15 components less than 5 μg/g.Four sesqueterpenoid alkaloids were firstly discovered from D.crepidatum.Alkaloids were mainly composed of octahydroindolizine alkaloids.

关 键 词：兰科 石斛 生物碱 倍半萜 八氢中氮茚 液质联用 

分 类 号：S567.23[农业科学—中草药栽培] R657[农业科学—作物学]