球孢白僵菌、少根根霉和产黄青霉对广藿香醇生物转化的作用研究  

Biotransformation of Patchoulol by Beauveria bassiana,Rhizopus arrhizu and Penicillium chrysogenum

作　　者：徐方方 韩晓东 张伟铮 漆睿 刘博 XU Fangfang;HAN Xiaodong;ZHANG Weizheng;QI Rui;LIU Bo(The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China)

机构地区：[1]广州中医药大学第二附属医院,广东广州510006

出　　处：《中药新药与临床药理》2020年第3期342-345,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家重点研发计划(2018YFC1707900);国家自然科学基金(81703379,81202398);广东省中医药局科研项目(20181121,20191142);广东省中医院中医药科技专项基金(YN2016QJ05);广州中医药大学一流学科重点项目(XK2018019);广州市科技计划项目(201607010338);广东省科技计划项目(2015A020211025)。

摘　　要：目的应用生物转化法对广藿香醇的进行羟基化修饰。方法选择真菌球孢白僵菌、少根根霉和产黄青霉对广藿香醇进行生物转化。转化产物经过凝胶、硅胶柱层析及制备型高效液相色谱法分离纯化,进而采用MS、NMR技术鉴定转化产物结构。结果广藿香醇经球孢白僵菌转化得到3个产物,分别为12-羟基广藿香醇(1)、8S-羟基广藿香醇(2)以及5R-羟基广藿香醇(3);经少根根霉转化得到3个产物,分别为3R-羟基广藿香醇(4)、8S-羟基广藿香醇(2)以及5R-羟基广藿香醇(3);经产黄青霉转化得到2个产物,分别为6R-羟基广藿香醇(5)和7S-羟基广藿香醇(6)。结论化合物1~6均为广藿香醇的羟基化衍生物。Objective To achieve hydroxylation of the patchoulol through biotransformation approach.Methods The fungi Beauveria bassiana,Rhizopus arrhizu and Penicillium chrysogenum were chosen as the biocatalysts for biotransformation of patchoulol.The products were extracted and purified using Sephadex LH20,silica column chromatography and semi-preparative HPLC,and subjected to EI-MS and NMR analysis to identify the structures.Results Three transformation products 12-OH patchoulol(1),8 S-OH patchoulol(2)and 5 R-OH patchoulol(3)were transfromed by B.bassiana;3 R-OH patchoulol(4),8 S-OH patchoulol(2)and 5 R-OH patchoulol(3)were transformed by R.arrhizu;6 R-OH patchoulol(5)and 7 S-OH patchoulol(6)were transformed by P.chrysogenum.Conclusion The products 1-6 were all identified as hydroxylated patchoulol.

关 键 词：广藿香醇 生物转化 真菌 羟基化 衍生物 

分 类 号：R284.1[医药卫生—中药学]