定志益聪颗粒对血管性痴呆大鼠学习记忆能力及Aβ1-42表达的影响  

Effect of Dingzhi Yicong Granule(定志益聪颗粒)on the Improvement of Learning and Memory Ability and the Expression of Abeta1-42 in Vascular Dementia Rats

作　　者：从琳[1] 杨秀娟[2] 张智龙[2] 王栩[2] 杨元庆[2] CONG Lin;YANG Xiu-juan;ZHANG Zhi-long;WANG Xu;YANG Yuan-qing(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital,Tianjin 300120,China)

机构地区：[1]天津中医药大学,天津301617 [2]天津市中医药研究院附属医院,天津300120

出　　处：《中医药导报》2020年第6期13-18,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：天津市卫生计生委、天津市中医药管理局资助项目(2017027)。

摘　　要：目的:探讨定志益聪颗粒对血管性痴呆(Va D)大鼠学习记忆能力及海马β淀粉样肽1-42(Aβ1-42)表达的影响。方法:SPF级雄性SD大鼠,随机分为正常组、假手术组、模型组、多奈哌齐组、定志益聪颗粒组。运用双侧颈总动脉闭塞(BCCAO)法制备Va D模型,于造模后56 d开始给药,正常组、假手术组、模型组予0.9%氯化钠注射液,多奈哌齐组(2.6×10-4g/kg)、定志益聪颗粒组(5 g/kg)均按10 mL/kg灌胃给药,1次/d,连续给药30 d。Morris水迷宫(MWM)考察大鼠空间学习、记忆能力。刚果红(CR)染色观察大鼠海马CA1区淀粉样变性;蛋白质免疫印迹法(Western blot)检测大鼠海马组织Aβ1-42的表达;免疫组化法(IHC)检测大鼠海马CA1区Aβ1-42的阳性表达。结果:与模型组比较,定志益聪颗粒组大鼠在定位航行试验中逃避潜伏期缩短(P<0.05),空间探索试验中经过平台次数、第Ⅰ象限停留时间、第Ⅰ象限路程增加(P<0.05),海马组织及海马CA1区Aβ1-42的表达减少(P<0.05)。结论:定志益聪颗粒可能通过下调海马Aβ1-42的表达,从而改善VaD大鼠的学习记忆能力。Objective:To investigate the effect of Dingzhi Yicong Granule(DZYCG)on learning and memory ability of vascular dementia(Va D)rats and its effect on expression of beta-amyloid peptide1-42(Aβ1-42)in hippocampus.Methods:SPF male SD rats were randomly divided into normal group,sham operation group,model group,donepezil group and DZYCG group.The Va D rat models were established by bilateral common carotid artery occlusion(BCCAO).The drugs were given in 56 days after model establishment.The normal group,sham operation group and model group were given 0.9%normal saline;Donepezil group(2.6×10-4g/kg)and DZYCG group(5 g/kg)were given corresponding drugs.The intragastric dose was 10 m L/kg body weight coefficient once a day for 30 days.Morris water maze(MWM)was used to investigate the spatial learning and memory ability of rats.Congo red(CR)staining for amyloidosis in hippocampal CA1 of rats.Western blot was used to detect the expression of Aβ1-42in hippocampal,and immunohistochemistry(IHC)was used to detect the positive expression of Aβ1-42in hippocampal CA1 of rats.Results:Compared with model group,the escaping latency of rats in DZYCG group was shortened(P<0.05),and the number of passing platform,the first quadrant residence time and the first quadrant distance increased(P<0.05);the expression of Aβ1-42in hippocampal and hippocampal CA1 decreased(P<0.05).Conclusion:DZYCG may improve the learning and memory ability of Va D rats by down-regulating the expression of Aβ1-42in hippocampal.

关 键 词：定志益聪颗粒 血管性痴呆 多奈哌齐 双侧颈总动脉闭塞法 刚果红染色 学习记忆能力 β淀粉样肽1-42 大鼠 

分 类 号：R285.5[医药卫生—中药学]