辽宁省间日疟原虫裂殖子表面蛋白1基因型及同源性分析  

A genotype and homology analysis of Plasmodium vivax merozoite surface protein 1 in Liaoning province,China

作　　者：王博[1] 李鑫[1] 毛玲玲[1] 孙英伟[1] 王子江[1] 于维君[1] 姚文清[1] WANG Bo;LI Xin;MAO Ling-ling;SUN Ying-wei;WANG Zi-jiang;YU Wei-jun;YAO Wen-qing(Liaoning Provincial Center for Disease Control and Prevention,Shenyang 110005,Liaoning Province,China)

机构地区：[1]辽宁省疾病预防控制中心感染与传染性疾病防制所,辽宁沈阳110005

出　　处：《中国媒介生物学及控制杂志》2020年第2期194-198,共5页Chinese Journal of Vector Biology and Control

基　　金：国家科技重大专项(2017ZX10103007-001)。

摘　　要：目的分析近10年辽宁省输入性及本地感染间日疟病例的疟原虫裂殖子表面蛋白1(PvMSP-1),并对其进行基因型别BLAST比对和同源性进化树分析。方法收集2008-2018年的间日疟病例样本,提取疟原虫基因组DNA,根据基因ICB5-ICB6区间片段设计引物,进行巢氏PCR扩增、测序、序列的比对以及同源性分析。结果共分析31份间日疟核酸样本,其中Sal-1型有13份,片段位置在470 bp左右,Belem型有18份,片段位置在400 bp左右。将所得出的目标产物送上海维基基因测序公司进行测序,经进化树的比对分析,31份样本中Sal-1型有13份,Belem型有5份,Ⅲ型的样本有13份。PvMSP-1基因ICB5-ICB6片段存在高度多态性,DNA序列可以结合流行病学调查来确定感染的来源。结论辽宁省在近10年当中输入性及本地感染的PvMSP-1基因型有Sal-1型、Ⅲ型和Belem型,优势基因型为Sal-1型和Ⅲ型。Objective To analyze Plasmodium vivax merozoite surface protein 1(PvMSP-1)in imported cases and local cases in Liaoning province,China in the past ten years,and to perform a genotype comparison of PvMSP-1(using BLAST)and homology analysis of the phylogenetic tree.Methods Plasmodium vivax samples were collected from cases during 2008-2018.The genomic DNAs of P.vivax were extracted and subjected to nested polymerase chain reaction-based amplification,sequencing,sequence alignment,and homology analysis using the primers designed based on the ICB5-ICB6 fragment.Results A total of 31 nucleic acid samples(13 Sal-1〔located around position 470 bp〕samples and 18 Belem〔located around position 400 bp〕samples)of P.vivax were analyzed.The resulting target products were sent to Shanghai Omicsspace Biotech Co.,Ltd.for sequencing.The phylogenetic tree comparison analysis revealed that the 31 samples were comprised of 13 Sal-1 samples,5 Belem samples,and 13 typeⅢsamples.The ICB5-ICB6 fragment of the PvMSP-1 gene was highly polymorphic,and the source of infection could be determined by combining the DNA sequences and the epidemiological investigation.Conclusion The genotypes of PvMSP-1 in imported and local cases in Liaoning province in the past ten years are types Sal-1,Ⅲ,and Belem,with the former two as the dominant types.

关 键 词：间日疟 裂殖子表面蛋白 多态性分析 

分 类 号：R382.31[医药卫生—医学寄生虫学]