卡介菌多糖核酸注射液辅助ALA-PDT治疗尖锐湿疣疗效及对患者血清LTN TGF-β1免疫功能细胞周期调节通路的影响  

Effect of BCG polysaccharide nucleic acid injection combined with ALA-PDT in the treatment of condyloma acuminatum and its effect on serum LTN,TGF-β1,immune function,and cell cycle regulation pathway

作　　者：贾雪平[1] 居兴刚[1] 杨元元[1] 陈琳[1] 姚莹[1] Jia Xueping;Ju Xinggang;Yang Yuanyuan;Chen Lin;Yao Ying(Second Affiliated Hospital of Henan University of Science and Technology,Luoyang 471000,Henan,China)

机构地区：[1]河南科技大学第二附属医院,河南洛阳471000

出　　处：《临床心身疾病杂志》2020年第2期15-20,共6页Journal of Clinical Psychosomatic Diseases

摘　　要：目的探讨卡介菌多糖核酸注射液辅助5-盐酸氨酮戊酸光动力疗法治疗尖锐湿疣患者的疗效和安全性,以及对血清淋巴细胞趋化因子、转化生长因子β1水平、免疫功能、细胞周期调节通路有丝分裂抑制因子p16、G1/S-特异性周期蛋白-D1、人视网膜母细胞瘤抑制蛋白pRb表达的影响.方法将60例尖锐湿疣患者按简单随机化分组法分为两组,每组30例,均予以5-盐酸氨酮戊酸光动力疗法治疗,观察组在此基础上予以卡介菌多糖核酸注射液治疗8周,治疗后观察1a.比较两组治愈率、复发率,治疗前后检测两组血清淋巴细胞趋化因子、转化生长因子β、全血CD3^+、CD4^+、CD8^+、CD4^+/CD8^+水平及细胞周期调节通路有丝分裂抑制因子p16、G1/S-特异性周期蛋白-D1、人视网膜母细胞瘤抑制蛋白pRb表达,随时记录治疗后出现的不良反应.结果(1)观察组治愈率(96.7%)显著高于对照组(73.3%),复发率(3.3%)显著低于对照组(26.7%)(P<0.05).(2)治疗后两组血清淋巴细胞趋化因子、转化生长因子β1及CD3^+、CD4^+、CD4^+/CD8^+水平较治疗前显著升高(P<0.01),观察组各项指标显著高于对照组(P<0.05或0.01);两组有丝分裂抑制因子p16、人视网膜母细胞瘤抑制蛋白pRb表达均较治疗前显著升高(P<0.01),观察组显著高于对照组(P<0.01),两组全血CD8^+水平、G1/S-特异性周期蛋白-D1表达均较治疗前显著降低(P<0.01),观察组显著低于对照组(P<0.01).(3)观察组不良反应发生率(6.7%)与对照组(3.3%)比较差异无统计学意义(P>0.05).结论卡介菌多糖核酸注射液辅助5-盐酸氨酮戊酸光动力疗法治疗尖锐湿疣患者具有协同增效作用,可提高治愈率,减少疾病复发,增强患者免疫功能,安全性高,下调G1/S-特异性周期蛋白-D1表达,上调有丝分裂抑制因子p16、人视网膜母细胞瘤抑制蛋白pRb、淋巴细胞趋化因子、转化生长因子β1表达可能是其发挥疗效的作用机制.Objective To explore the effect of BCG polysaccharide nucleic acid injection combined with ALA-PDT in the treatment of condyloma acuminatum and its effect on serum LTN,TGF-β1,immune function,and cell cycle regulation pathway mitosis inhibiting factorP16,G1/S-specific cyclin-D1,human retinoblastoma inhibitory protein pRb expression.Methods 60 patients with CA were randomly divided into two groups,30 cases each.The control group was treated with ALA PDT,while the observation group was treated with BCG polysaccharide nucleic acid injection.The patients were treated for 8 weeks,and observed for 1 year after treatment.The cure rate,recurrence rate,serum LTN,TGF-β1,whole blood CD3^+,CD4^+,CD8^+,CD4^+/CD8^+levels,cell cycle regulation pathway mitosis inhibiting factorP16,G1/S-specific cyelin-D1,human retinoblastoma inhibitory protein pRb expression were compared between the two groups.Adverse reactions after treatment were recorded at any time.Results(1)The cure rate(96.7%)in the observation group was significantly higher than that in the control group(73.3%),and the recurrence rate(3.3%)was significantly lower than in the control group(26.7%)(P<0.05).(2)Serum LTN,TGF-β1 and CD3^+,CD4^+,CD4^+/CD8^+levels.were significantly higher in the two groups after treatment than before treatment(P<0.01),and the indicators in the observation group were significantly higher than those in the control group(P<<0.05 or 0.01).The expressions of mitosis inhibiting factor p16 and human retinoblastoma inhibitory protein pRb in the two groups were significantly higher than before treatment(P<0.01).The observation group was significantly higher than the control group(P<0.01).The expression of whole blood CD8^+and G1/S-specific CyclinD1 in both groups was significantly lower than that before treatment(P<0.01),and the observation group was significantly lower than the control group(P<0.01).(3)There was no significant difference in the incidence of adverse reactions between the observation group(6.7%)and the control group(3.3%)(P>0.05).Concl

关 键 词：尖锐湿疣 卡介菌多糖核酸注射液 5-盐酸氨酮戊酸 光动力疗法 临床疗效 复发率 淋巴细胞趋化因子 转化生长因子Β1 免疫功能 细胞周期调节通路 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]