Elicitor hydrophobin Hyd1 interacts with Ubiquilin1-like to induce maize systemic resistance  

作　　者：Chuanjin Yu Kai Dou Shaoqing Wang Qiong Wu Mi Ni Tailong Zhang Zhixiang Lu Jun Tang Jie Chen 

机构地区：[1]Key Laboratory of Urban Agriculture(South)Ministry of Agriculture,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [2]State Key Laboratory of Microbial Metabolism,Shanghai Jiao Tong University,Shanghai 200240,China [3]School of Life Science,Fuyang Normal University,Fuyang 236037,China

出　　处：《Journal of Integrative Plant Biology》2020年第4期509-526,共18页植物学报（英文版）

基　　金：supported by the grant from National Key Projects of I Intergovernmental Cooperation in Scientific and Technological Innovation(2017YFE 0104900);the National Natural Science Foundation of China(31872015,31672072);National Key Research and Development Program of China(2017YFD0200901);Agriculture Research System of Shanghai(Grant No.201710);China Agriculture Research System Project(CARS-02)。

摘　　要：Trichoderma harzianum is a plant-beneficial fungus that secretes small cysteine-rich proteins that induce plant defense responses;however, the molecular mechanism involved in this induction is largely unknown.Here, we report that the class Ⅱ hydrophobin Th Hyd1 acts as an elicitor of induced systemic resistance(ISR) in plants. Immunogold labeling and immunofluorescence revealed Th Hyd1 localized on maize(Zea mays) root cell plasma membranes. To identify host plant protein interactors of Hyd1, we screened a maize B73 root c DNA library. Th Hyd1 interacted directly with ubiquilin1-like(UBL). Furthermore, the N-terminal fragment of UBL was primarily responsible for binding with Hyd1 and the eight-cysteine amino acid of Hyd1 participated in the protein-protein interactions. Hyd1 from T. harzianum(Thhyd1) and ubl from maize were co-expressed in Arabidopsis thaliana, they synergistically promoted plant resistance against Botrytis cinerea. RNA-sequencing analysis of global gene expression in maize leaves 24 h after spraying with Curvularia lunata spore suspension showed that Thhyd1-induced systemic resistance was primarily associated with brassinosteroid signaling, likely mediated through BAK1. Jasmonate/ethylene(JA/ET)signaling was also involved to some extent in this response. Our results suggest that the Hyd1-UBL axis might play a key role in inducing systemic resistance as a result of Trichoderma-plant interactions.

关 键 词：ZEA mays resistance MAIZE 

分 类 号：S513[农业科学—作物学]