核致密斑点型抗核抗体的临床应用  

Clinical application of nuclear dense spot antinuclear antibody

作　　者：戴蕾[1] 臧福宇 朱文波[1] 郑齐锶 宁明哲[1] 陶月[1] DAI Lei;ZANG Fu-yu;ZHU Wen-bo;ZHENG Qi-si;NING Ming-zhe;TAO Yue(Department of Clinical Laboratory,Affiliated Drum Tower Hospital of Nanjing University Medical College,Nanjing 210008,Jiangsu,China)

机构地区：[1]南京大学医学院附属鼓楼医院检验科,南京210008

出　　处：《东南国防医药》2020年第3期268-271,共4页Military Medical Journal of Southeast China

摘　　要：目的探讨核致密斑点型(AC-2)自身抗体的临床应用。方法收集19例南京大学医学院附属鼓楼医院检验科的血清标本,免疫印迹法(WB)检测抗核抗体谱(IgG)和抗DFS70抗体(IgG),间接免疫荧光法(IIF)检测抗核抗体(IgG),观察并记录结果。结果19例标本中,抗DFS70抗体均为阳性,抗核抗体核型均为AC-2,抗核抗体谱中有2例阳性。结论AC-2是荧光模型国际共识(ICAP)定义的细胞核核型中的斑点型的亚型之一,国内检验人员应努力学习其荧光显微镜下表现,了解其临床意义,为临床提供准确的检验报告。Objective To investigate the clinical application of nuclear dense fine speckled(AC-2)autoantibodies.Methods 19 serum samples were collected from the Laboratory Department of Gulou Hospital Affiliated to Medical College of Nanjing University.Antinuclear antibody spectrum(IgG)and anti-DFS70 antibody(IgG)were detected by immuno-blotting method,and anti-nuclear antibody(IgG)was detected by indirect immunofluorescence method.The results were observed and recorded.Results Anti-DFS70 antibody was positive in all 19 specimens,anti-nuclear antibody karyotype was dense spot type(AC-2),and anti-nuclear antibody spectrum was positive in 2 of 19 specimens.Conclusion AC-2 is one of the dot subtypes in the karyotype defined by ICAP.Domestic laboratory personnel should study its fluorescence microscopic manifestations,understand its clinical significance and provide accurate test reports for clinical practice.

关 键 词：抗核抗体 HEP-2细胞 间接免疫荧光 抗DFS70抗体 致密斑点型 

分 类 号：R446.6[医药卫生—诊断学]