三维打印聚乳酸支架促进幼年小鼠体外骨样基质沉积的实验研究  

Experimental study on the promotion of bone matrix deposition in vitro by 3D printing polylactic acid scaffolds in young mice

作　　者：杨轩 楼跃 郑鹏飞 YANG Xuan;LOU Yue;ZHENG Peng-fei(Department of Pediatric Orthopedics,Children's Hospital of Nanjing Medical University,Nanjing Jiangsu 210000,China)

机构地区：[1]南京医科大学附属儿童医院儿骨科,江苏南京210000

出　　处：《临床和实验医学杂志》2020年第8期789-792,共4页Journal of Clinical and Experimental Medicine

基　　金：江苏省卫计委医学科研课题(编号:H2018111)。

摘　　要：目的 研究三维打印聚乳酸支架促进幼年小鼠体外骨样基质沉积的效果。方法 取小白鼠额、顶骨,经细胞分离培养小鼠成骨细胞,然后与三维打印聚乳酸支架、白磷钙石(WH)复合材料支架、纳米有机复合材料支架分别培养,分别计算各组12 h、24 h、36 h、48 h成骨细胞粘附率、增殖率、骨桥蛋白(OPN)基因表达水平、骨钙素分泌情况以及骨基质沉淀率。结果 三维打印聚乳酸支架细胞粘附率、成骨细胞增殖率、细胞骨基质沉淀率显著高于纳米有机复合支架和白磷钙石复合支架(均P <0. 05),且培养时间从12 h延长至48 h,三组以上指标也均显著增加(均P <0. 05);与白磷钙石复合支架组相比,各时间点三维打印聚乳酸支架组细胞OPN基因表达水平和骨钙素分泌量均显著增加(P<0. 05),但与纳米有机复合支架组相比,仅培养36 h时三维打印聚乳酸支架组细胞OPN基因表达水平显著增加(P <0. 05),仅培养48 h时三维打印聚乳酸支架组骨钙素分泌量显著增加(P <0. 05);随着时间延长,三组细胞OPN基因表达水平和骨钙素分泌量也均显著增加(P <0. 05)。结论 三维打印聚乳酸支架具有促进幼年小鼠体外骨样基质沉积的作用,其总体作用效果最显著培养时长为36~48 h,因此值得在临床骨缺损修复中推广使用。Objective To study the effect of 3D printing polylactic acid scaffold on bone matrix deposition in vitro in young mice.Methods Mice frontal and parietal cells were selected,and osteoblasts were cultivated and combined cultivated with 3D printing polylactic acid scaffold,white phosphorite(WH)composite stents,stent combined training of organic composite materials.The osteoblast adhesion rate,proliferation rate,OPN gene expression level,osteocalcin secretion and bone matrix deposition rate at 12 h,24 h,36 h and 48 h were calculated.Results 3D printing polylactic acid scaffolds had significantly higher cell adhesion rate,osteoblast proliferation rate,and cell bone matrix sedimentation rate than that of the nano-organic composite scaffolds and white apatite composite scaffolds(P<0.05),and with the extension of time from 12 h to 48 h,the indexes in the three groups also increased significantly(P<0.05).Compared with the white apatite composite scaffold group,the expression levels of OPN gene and osteocalcin secretion in the 3D printing polylactic acid scaffold group at each time point were significantly increased(P<0.05).However,compared with the nano-organic composite scaffold group,the OPN gene expression level of the 3D printing polylactic acid scaffold group was significantly increased when cultured only for 36 hours(P<0.05),and the bones of the 3D printing polylactic acid scaffold group were cultured for only 48 h.Calcium secretion increased significantly(P<0.05).With the extension of time,OPN gene expression levels and osteocalcin secretion in the three groups of cells also increased significantly(P<0.05).Conclusion 3D printing polylactic acid scaffolds can promote the deposition of osteoid matrix in vitro in young mice,and the culture time of most significant overall effect is from 36 h to 48 h,so it is worthy of extensive clinical application in bone defect repair.

关 键 词：小鼠 体外骨样基质沉积 三维打印聚乳酸支架 

分 类 号：R318.08[医药卫生—生物医学工程] TP391.73[医药卫生—基础医学]