晚期糖基化终产物诱导小鼠胰岛素抵抗的研究  

The Study of the Induction of Insulin Resistance in Mice by Advanced Glycation End Products

作　　者：董璐 吴玥琨 吴亚敬 张燕 DONG Lu;WU Yue-kun;WU Ya-jing;ZHANG Yan(State Key Laboratory of Food Nutrition and Safety,College of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Tianjin Key Laboratory of Food Science and Health,School of Medicine,Nankai University,Tianjin 300071,China)

机构地区：[1]天津科技大学食品工程与生物技术学院,食品营养与安全国家重点实验室,天津300457 [2]南开大学医学院,天津市食品科学与健康重点实验室,天津300071

出　　处：《食品研究与开发》2020年第10期1-5,共5页Food Research and Development

基　　金：国家重点研发计划课题(2016YFD0400401)。

摘　　要：以丙酮醛修饰β-乳球蛋白产物(methylglyoxal modifiedβ-lactoglobulin product,MG-bLG)为蛋白质结合态晚期糖基化终产物(advanced glycation end products,AGEs)的代表物,通过灌胃摄入方式研究AGEs对小鼠胰岛素抗性的影响。经长时间摄入MG-bLG,相较于对照组(C组),实验组(MG-bLG组)小鼠摄食量增多,体重增大,血清中低密度脂蛋白、总胆固醇和甘油三酯含量显著增高(P<0.05),空腹血糖显著升高(P<0.05)至7.1 mmol/L,空腹胰岛素水平显著升高(P<0.05)至14.8 mU/L,胰岛抵抗指数和胰岛β细胞功能指数同样显著升高(P<0.05)。此外,MG-bLG组小鼠血清中AGEs水平显著升高(P<0.05)至131.99 pg/mL。因此,长期摄入AGEs可改变机体的糖脂代谢情况,促进机体胰岛素抵抗形成,增加机体患2型糖尿病风险。The methylglyoxal-modified β-lactoglobulin product(MG-bLG)was used as a representative of the protein-bound advanced glycation end products(AGEs)to study the effect of protein-bound AGEs on insulin resistance(IR)in mice by intragastric ingestion. After long-term intake of MG-bLG,compared with the control group(group C),the experimental group(MG-bLG group)increased the food intake and weight,the content of serum low-density lipoprotein,total cholesterol and triglycerides were significantly increased(P<0.05),the fasting blood glucose was significantly increased to 7.1 mmol/L(P<0.05),the fasting insulin level was significantly increased to 14.8 mU/L(P<0.05),homeostasis model assessment-insulin resistance index was significantly increased(P<0.05),homeostasis model assessment-insulin sensitivity index was significantly decreased(P<0.05). In addition,the serum AGEs levels in the MG-bLG group were significantly increased to 131.99 pg/mL(P<0.05). Therefore,long-term intake of AGEs can change the glucose-lipid metabolism,promote the formation of insulin resistance,and increase the risk of type 2 diabetes in the body.

关 键 词：蛋白质结合态晚期糖基化终产物 膳食摄入 丙酮醛修饰β-乳球蛋白产物 糖脂代谢 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]