人参皂苷Rg1对K562细胞增殖的影响  

Effects of Ginsenoside Rg1 on the Proliferation of K562 Cells

作　　者：韩艳军 王翠丽 刘小虎 周玥 Han Yanjun;Wang Cuili;Liu Xiaohu;Zhou Yue(Pre-clinical College,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学基础医学院,云南大理671000

出　　处：《大理大学学报》2020年第4期16-20,共5页Journal of Dali University

基　　金：国家自然科学基金资助项目(81660731,81860038)。

摘　　要：目的:探讨人参皂苷Rg1对K562细胞增殖的影响。方法:采用不同浓度的人参皂苷Rg1处理K562细胞,用CCK-8法和集落生成实验检测其对K562细胞增殖的影响;用流式细胞术检测K562细胞周期。结果:人参皂苷Rg1能够有效抑制K562细胞增殖,降低K562细胞集落形成率,抑制K562细胞进入S期,并阻滞在G0/G1期。对K562细胞增殖抑制效果最强的浓度是20μmol/L,最佳作用时间是48 h。结论:人参皂苷Rg1能够有效抑制K562细胞增殖。Objective:To investigate the effects of Ginsenoside Rg1 on the proliferation of K562 cells.Methods:K562 cells were treated with different concentrations of Ginsenoside Rg1.The effects of Ginsenoside Rg1 on proliferation of K562 cells were detected by CCK-8 assay and colony formation assay.The cell cycle of K562 was detected by flow cytometry.Results:Ginsenoside Rg1 could effectively inhibit the proliferation of K562 cells,decrease the colony formation rate of K562 cells,inhibit K562 cells from entering S phase,and block G0/G1 phase.The most potent inhibitory effect on K562 cell proliferation was 20μmol/L,and the optimal duration of action was 48 h.Conclusion:Ginsenoside Rg1 can effectively inhibit the proliferation of K562 cells.

关 键 词：人参皂苷RG1 K562细胞 细胞增殖 细胞周期 

分 类 号：R285[医药卫生—中药学]