苯甲酰芍药苷增强ABCA1表达促进胆固醇流出  

Benzoylpaeoniflorin promote cholesterol efflux by enhancing ABCA1 expression

作　　者：陈晓佳 唐菀泽 马卫列 丁航 张志珍 CHEN Xiaojia;TANG Wanze;MA Weilie;DING Hang;ZHANG Zhizhen(School of Basic Medicine,Guangdong Medical University,Guangdong Province,Dongguan 523808,China;Department of Spine Surgery,Shenzhen People′s Hospital Shenzhen Institute for Orthopaedic Research,Guangdong Province,Shenzhen 518020,China)

机构地区：[1]广东医科大学基础医学院,广东东莞523808 [2]深圳市人民医院脊柱外科深圳市骨科研究所,广东深圳518020

出　　处：《中国医药导报》2020年第10期8-12,共5页China Medical Herald

基　　金：广东省中医药局中医药科研项目(20191188)。

摘　　要：目的探讨白芍醇提物苯甲酰芍药苷(BP)对THP-1细胞胆固醇流出的影响。方法实验分ac-LDL组、apoA-1组和apoA-1+BP组。THP-1细胞加入160 nmol/L佛波酯(PMA)24 h,再加入50μg/mL乙酰化低密度脂蛋白(ac-LDL)建立泡沫细胞模型。液体闪烁计数法测定泡沫细胞胆固醇流出率;酶化学反应检测细胞总胆固醇浓度;定量PCR(qPCR)检测三磷酸腺苷结合盒转运体A1(ABCA1)表达;Western blot分析ABCA1、固醇调节元件结合蛋白(SREBPs)、肝X受体α(LXRα)蛋白表达变化。结果成功建立THP-1泡沫细胞模型。apoA-1+BP组泡沫细胞胆固醇流出率高于apoA-1组,差异有高度统计学意义(P <0.01)。apoA-1+BP组泡沫细胞内总胆固醇含量低于apoA-1组,差异有高度统计学意义(P <0.01)。apoA-1+BP组泡沫细胞ABCA1 mRNA和蛋白表达量均高于apoA-1组,差异有统计学意义(P <0.05或P <0.01)。apoA-1+BP组泡沫细胞LXRα蛋白表达水平高于apoA-1组,差异有高度统计学意义(P <0.01)。apoA-1+BP组SREBP1、SREBP2蛋白表达量低于apoA-1组,差异有高度统计学意义(P <0.01)。结论 BP可通过LXRα-ABCA1通路促进胆固醇流出。Objective To investigate the effect of Benzoylpaeoniflorin(BP) on cholesterol efflux from THP-1 cells.Methods The experiment was divided into ac-LDL group, apoA-1 group and apoA-1+BP group. The model of foam cells was established by adding 160 nmol/L phorbol-1-myristate-13-acetate(PMA) for 24 h in THP-1 cells, followed by 50 μg/mL acetylated low-density lipoprotein(ac-LDL). The foam cells cholesterol efflux rate was determined using liquid scintillation counting, the contents of total cholesterol of foam cells were observed by enzymatic reaction method.adenosine triphosphate binding cassette transporter A1(ABCA1) m RNA expression was detected by quantitative polymerase chain reaction(qPCR). The expression of ABCA1, sterol regulated original binding proteins(SREBPs)and Liver X receptor alpha(LXRα) were analyzed by Western blot. Results THP-1 cell model was successfully established.Cholesterol efflux of foam cells in apoA-1 +BP group was higher than that in apoA-1 group, and the difference was highly statistically significant(P < 0.01). The total cholesterol content of foam cells in apoA-1+BP group were lower than those in apoA-1 group, and difference was highly statistically significant(P < 0.01). The mRNA and protein levels of ABCA1 in apoA-1+BP group were higher than those in apoA-1 group, and the difference was statistically significant(P < 0.05 or P < 0.01). The expression levels of LXRα in apoA-1+BP group were higher than those in apoA-1 group,and the difference was highly statistically significant(P < 0.01). The SREBP1 and SREBP2 expression levels in apoA-1 +BP group were lower than those in apoA-1 group,and the difference was highly statistically significant(P <0.01). Conclusion BP can promote cholesterol efflux through the LXRα-ABCA1 pathway.

关 键 词：苯甲酰芍药苷 胆固醇流出 泡沫细胞 

分 类 号：R282[医药卫生—中药学] Q54[医药卫生—中医学]