UHPLC-MS/MS法测定大鼠血浆中硼替佐米的含量  

Determination of Bortezomib in rat plasma by UHPLC-MS/MS method

作　　者：刘进 柳珂[2] 蒋巍 黄翠云 邓易 束畅 张凤 LIU Jin;LIU Ke;JIANG Wei;HUANG Cuiyun;DENG Yi;SHU Chang;ZHANG Feng(Department of Hematology,Shanghai Changzheng Hospital,Shanghai 200003,China;Department of Oncology,Shanghai Changzheng Hospital,Shanghai 200003,China;Department of Pharmacy,Shanghai Changzheng Hospital,Shanghai 200003,China)

机构地区：[1]上海长征医院血液科,上海200003 [2]上海长征医院肿瘤科,上海200003 [3]上海长征医院药材科,上海200003

出　　处：《中国医药导报》2020年第10期22-25,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81573793)。

摘　　要：目的建立超高效液相色谱-质谱联用技术检测硼替佐米的方法,并用于大鼠血浆硼替佐米浓度的测定。方法采用蛋白沉淀法处理血浆样品,色谱柱为Waters XBridge BEH C18柱(2.5μm,2.1 mm×50 mm),以乙腈-醋酸铵(10 mmol/L)为流动相梯度洗脱,流速为0.3 mL/min,柱温为35°C,多反应检测正离子模式分析。结果硼替佐米血浆浓度在0.5~100 ng/mL线性范围内良好,标准曲线回归方程为Y=14.41X-0.4490(r=0.9974)。定量下限为0.5 ng/mL,低、中、高3个质量浓度的质控样品日内、日间精密度和准确度、提取回收率、基质效应和稳定性结果均良好。结论该法准确、灵敏、重现性好,可用于大鼠硼替佐米药效动力学研究。Objective To establish an ultra high performance liquid chromatography-tandem mass spectrometry method for the determination of Bortezomib in rat plasma.Methods Plasma sample preparation was treated by protein precipitation.ColumWaters ■BEH C18(2.5μm,50 mm×2.1 mm)was used as the column.Acetonitrile and 10 mmol/L ammonium formate solution was used as the mobile phase,with gradient elute,the volume flow rate was 0.3 mL/min the column temperature was maintained at at 35℃.Multi-reaction detection positive ion mode was used to analysis Results The Bortezomib in the range of 0.5-100 ng/mL showed a good liner,the standard linear regression equation was Y=14.41X-0.4490(r=0.9974).The lower limit of quantification was 0.5 ng/mL,and the results of in-day,day-time precision and accuracy,extraction recovery,matrix effect and stability of quality control samples with low,medium and high 3 mass concentrations were all good.Conclusion The method is accurate,sensitive and reproducible,and can be used in the pharmacokinetic study of Bortezomib in rats.

关 键 词：超高效液相色谱-质谱联用法 硼替佐米 血浆 药效动力学 

分 类 号：R971.1[医药卫生—药品]