蒙药斯日西防治实验性胃溃疡作用及机制研究  

Study on the effect and mechanism of Mongolian medicine Sirixi in preventing and treating experimental gastric ulcer

作　　者：敖登其木格 王海荣 乌汉其木格 特格喜白音 Aodengqimuge;WANG Hairong;Wuhanqimuge;Tegexibaiyin(Innovative Mongolian Pharmaceutical Preparation Engineering Laboratory,Inner Mongolia International Mongolian Hospital,Inner Mongolia Autonomous Region,Hohhot 010065,China)

机构地区：[1]内蒙古自治区国际蒙医医院创新蒙药制剂工程实验室,内蒙古呼和浩特010065

出　　处：《中国医药导报》2020年第12期23-27,共5页China Medical Herald

基　　金：内蒙古自治区蒙医药研究所蒙药新药研发及药物实验室的建设项目(2016YJS06);中共内蒙古自治区蒙医药研究所蒙药新药研发及产业化创新新团队项目(内组通字[2015]56号)。

摘　　要：目的研究蒙药斯日西对胃溃疡的防治作用及其可能的机制。方法选取25只Wistar大鼠,采用吲哚美辛诱导的急性胃溃疡模型,苏木精-伊红染色观察胃黏膜变化;选取25只昆明小鼠,采用乙醇/HCL诱导的急性胃溃疡模型,观察胃组织中髓过氧化物酶(MPO)活性,总谷胱甘肽(GSH)水平以及外周血T淋巴细胞水平的变化;选取20只Wistar大鼠,采用乙酸烧灼性慢性胃溃疡模型,酶联免疫吸附测定法检测胃组织中炎性因子肿瘤坏死因子-α(TNF-α)、细胞间黏附分子1(ICAM-1)表达水平。结果与模型组比较,斯日西散组胃黏膜病变减轻;与模型组比较,斯日西散组胃组织MPO活性、大鼠胃黏膜TNF-α、ICAM-1表达水平降低,GSH表达水平升高,差异均有高度统计学意义(均P <0.01);与模型组比较,斯日西散组胸腺、脾脏重量指数、外周血CD4+细胞表达水平升高,差异均有统计学意义(P <0.05或P <0.01),而两组CD3^+、CD4^+/CD8^+、CD8^+细胞表达水平比较,差异无统计学意义(P> 0.05)。结论斯日西散防治胃溃疡的作用机制可能是通过促进防御因子,抑制损害因素,提高免疫能力而实现的。Objective To study the preventive and therapeutic effect of Mongolian medicine Sirixi on gastric ulcer and its possible mechanism. Methods A total of 25 Wistar rats were selected to make rat acute gastric ulcer model induced by indomethacin. Changes of gastric mucosa were observed by hematoxylin-eosin staining. A total of 25 Kunming mice were selected to observe the changes of myeloperoxidase(MPO) activity, glutathione(GSH) level and peripheral blood T lymphocyte level in gastric tissues in the ethanol/HCL-induced acute gastric ulcer model. A total of 20 Wistar rats were selected and the expression levels of inflammatory tumor necrosis factor-α(TNF-α) and intercellular adhesion molecule 1(ICAM-1) in gastric tissues were determined by enzyme-linked immunosorbent assay in a acetic acid-burning chronic gastric ulcer model. Results Compared with the model group, the gastric mucosal lesions were alleviated in the Sirixi Powder group. Compared with the model group, the gastric tissue MPO activity, the expression levels of TNF-αand ICAM-1 in the gastric mucosa of the Sirixi Powder group were decreased, and the expression levels of GSH were increased, the differences were highly statistically significant(all P < 0.01). Compared with the model group, the thymus, spleen weight index and the expression levels of peripheral blood CD4+cells were increased in the Sirixi Powder group, with statistically significant differences(P < 0.05 or P < 0.01), while there was no significant difference in the expression levels of CD3^+, CD4^+/CD8^+and CD8+between the two groups(P > 0.05). Conclusion The mechanism of action of Sirixi Powder in the prevention and treatment of gastric ulcer may be realized by promoting defense factor, inhibiting damage factor and improving immune ability.

关 键 词：斯日西 髓过氧化物酶 总谷胱甘肽 T淋巴细胞 肿瘤坏死因子-α 细胞间黏附分子1 

分 类 号：R285.5[医药卫生—中药学]