基于UPLC-HDMS方法对健脾化湿法干预脾虚湿盛证肝癌患者的血浆代谢组学研究  

Study on plasma metabolomics of liver cancer patients with syndrome of dampness excessiveness due to spleen deficiency based on UPLC-HDMS

作　　者：王东[1] 李晶洁[1] 吕书勤[1] WANG Dong;LI Jingjie;LYU Shuqin(VIP Ward,Chinese Medicine Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi830000,China)

机构地区：[1]新疆医科大学附属中医医院VIP病房,新疆乌鲁木齐830000

出　　处：《中国医药导报》2020年第12期145-148,共4页China Medical Herald

基　　金：国家自然科学基金地区科学基金项目(81460689)。

摘　　要：目的采用代谢组学探究脾虚湿盛证肝癌患者发病机制及健脾化湿法干预的分子生物学效应。方法选取2017年6月~2019年6月新疆医科大学附属中医医院(以下简称"我院")体检中心体检的50名平和质者作为空白组,选取我院收治的50例脾虚湿盛证肝癌患者作为对照组,50例服用四君子汤干预1个月的肝癌患者作为观察组。采用超高效液相色谱-高清质谱联用技术对三组入选者血浆样本进行代谢组学分析。结果在入选对象血液样品中共鉴定出谷胱甘肽、左旋谷氨酸等19个潜在生物标志物。与空白组比较,对照组中有6个表现为上升趋势,13个表现为下降趋势(P <0.05或P <0.01)。经四君子汤干预后,其中17个具有回调作用,调整差异有统计学意义(P <0.05或P <0.01);根据这17个回调生物标志物找到D-谷氨酰胺和谷氨酸代谢、牛磺酸和牛磺酸代谢等6条相关代谢通路。结论脾虚湿盛证肝癌患者体内存在氨基酸代谢、花生四稀酸代谢、脂肪酸代谢、谷胱甘肽代谢等紊乱,四君子汤可能通过调节以上相关代谢通路对脾虚湿盛证肝癌患者具有调节作用。Objective To study the pathogenesis of liver cancer with syndrome of dampness excessiveness due to spleen deficiency by metabonomics and the molecular biological effect of the intervention of invigorating spleen for eliminating dampness. Methods Fifty persons with peace quality of blank group were selected from the physical examination center of Chinese Medicine Hospital of Xinjiang Medical University(hereinafter referred to as "our hospital") from June 2017 to June 2019. 50 cases of liver cancer patients with spleen deficiency and dampness syndrome in our hospital were taken as control group and 50 cases of liver cancer patients taking Sijunzi Decoction for one month were taken as observation group. Metabonomics analysis of plasma samples from three groups of participants was carried out by using the technology of ultra-high performance liquid chromatography-high definition mass spectrometry. Results Nineteen potential biomarkers such as glutathione and L-glutamic acid were identified in the blood samples of the selected subjects. Compared with blank group, 6 of control group showed an upward trend and 13 showed a downward trend(P <0.05 or P < 0.01). After intervention of Sijunzi Decoction, 17 of them had callback effect, and the adjustment difference was statistically significant(P < 0.05 or P < 0.01). According to these 17 callback biomarkers, 6 related metabolic pathways including D-glutamine and glutamate metabolism, taurine and taurine metabolism were found. Conclusion There are disorders of amino acid metabolism, arachidonic acid metabolism, fatty acid metabolism and glutathione metabolism in liver cancer of syndrome of dampness excessiveness due to spleen deficiency. Sijunzi Decoction may regulate the disorders by above metabolic pathways in liver cancer of syndrome of dampness excessiveness due to spleen deficiency.

关 键 词：肝癌 脾虚湿盛证 超高效液相色谱-高清质谱 血浆代谢组学 

分 类 号：R246.5[医药卫生—针灸推拿学]