木薯组培苗中叶绿素和类胡萝卜素含量分析  

Analysis of carotenoids chlorophyⅡ and in cassava tissue culture seedlings

作　　者：罗秀芹[1] 韦卓文 杨龙[1,2] 薛晶晶[1] LUO Xiu-qin;WEI Zhuo-wen;YANG Long;XUE Jing-jing(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences(CATAS)/Key Laboratory of Conservation and Utilization of Cassava Genetic Resources,Ministry of Agriculture and Rural Affairs,Haikou 571101,China;Subtropical Crop Institute of Guizhou Agricultural Science Academy,Xingyi 562400,Guizhou,China)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所/农业农村部木薯种质资源保护与利用重点实验室,海口571101 [2]贵州省农业科学院亚热带作物研究所,贵州兴义562400

出　　处：《湖北农业科学》2020年第4期136-140,共5页Hubei Agricultural Sciences

基　　金：农业部国家木薯种质资源保护与创新利用重点实验室运行费项目;中国热带农业科学院基本科研业务费专项基金项目(1630032018027)。

摘　　要：以TM60444和SC9木薯(Manihot esculenta Crantz)组培苗根、茎、叶为材料,采用乙醇∶丙酮溶液萃取方法,利用紫外-可见分光光度计对两种木薯组培苗根、茎、叶中的叶绿素和胡萝卜素含量进行了测定,并对类胡萝卜素生物合成相关基因进行了定量分析。结果表明,两个品种叶绿素和胡萝卜素含量都是叶>茎>根;而类胡萝卜素含量是叶>茎≈根。Ultraviolet-visible spectrophotometer was used to detect the content of chlorophy Ⅱ and carotenoids in TM60444 and SC9 tissues culture seedings. Meanwhile, the relative genes of carotenoids biosynthesis were analyzed. The results showed that there is nearly no difference between two stains and the contents of chlorophyll and total carotene in two seedings : leaf>stem>root;and the content of carotenoids: leaf>stem, while the stem is approximately the root.

关 键 词：木薯(Manihot esculenta Crantz) 类胡萝卜素 定量PCR 紫外-可见分光光度计 

分 类 号：S533[农业科学—作物学]