Effects of sodium nitroprusside on callus browning of Ficus religiosa: an important medicinal plant  

作　　者：Mohsen Hesami Masoud Tohidfar Milad Alizadeh Mohammad Hosein Daneshvar 

机构地区：[1]Department of Horticultural Science,Faculty of Agriculture,University of Tehran,Karaj,Iran [2]Department of Plant Biotechnology,Faculty of Life Science and Biotechnology,Shahid Beheshti University,Tehran,Iran [3]Department of Horticultural Science,Faculty of Agriculture,Khuzestan Agricultural Sciences and Natural Resources University,Mollasani,Iran

出　　处：《Journal of Forestry Research》2020年第3期789-796,共8页林业研究（英文版）

摘　　要：Tissue browning is a major problem in tissue culturing of woody plants, especially for Ficus religiosa which occurs by the accumulation and oxidation of phenolic compounds. This study aimed to determine the effect of different concentrations of sodium nitroprusside on the appearance of callus browning from leaf explants. The results indicate that callus browning was significantly reduced by supplementation of sodium nitroprusside to the MS medium and supplemented with 2.26μM of 2,4-dichlorophenoxyacetic acid and 0.22μM of 6-benzyl amino purine. The accumulation of hydrogen peroxide and phenolic compounds in the callus tissues decreased at the 50 μM concentration of sodium nitroprusside. Although catalase and peroxidase activities decreased at the 50μM concentration, the activity of superoxide dismutase and polyphenol oxidases, as well as proline content, increased exponentially. Sodium nitroprusside could be useful for the formation of non-embryogenic callus with high levels of metabolic activity for the production and isolation of secondary metabolites.

关 键 词：NITRIC oxide Oxidative enzymes PROLINE Reactive oxygen species 

分 类 号：S567.19[农业科学—中草药栽培]