双黄连片高效液相色谱指纹图谱的建立及聚类分析和主成分分析  

Establishment of HPLC Fingerprint,Cluster Analysis and Principal Component Analysis of Shuanghuanglian Tablets

作　　者：唐佩琴 TANG Peiqin(Department of Pharmacy,The 900th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army,Fuzhou,Fujian,China 350000)

机构地区：[1]中国人民解放军联勤保障部队第九○○医院药学科,福建福州350000

出　　处：《中国药业》2020年第9期102-105,共4页China Pharmaceuticals

摘　　要：目的建立双黄连片的高效液相色谱指纹图谱,并进行聚类分析和主成分分析。方法色谱柱为Agilent Zorbax-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.07%甲酸溶液(梯度洗脱),流速为0.9 mL/min,检测波长为320 nm(绿原酸和木犀草苷)、220 nm(连翘酯苷A和连翘苷)、275 nm(黄芩苷、汉黄芩苷及黄芩素),柱温为30℃,进样量为10μL。以连翘酯苷A峰为参照,绘制12批双黄连片的高效液相色谱指纹图谱,采用2012年版《中药色谱指纹图谱相似度评价系统》进行相似度评价,确定共有峰,并采用SPSS 22.0统计学软件进行聚类分析和主成分分析。结果建立了12批双黄连片的高效液相指纹色谱图谱,共确定18个共有峰,方法学考察结果符合指纹图谱的技术要求,相同厂家的药品聚为一类;经主成分分析,2个主成分因子的累计方差贡献率为92.242%,样品中绿原酸、木犀草苷、连翘酯苷A、连翘苷、黄芩苷、黄芩素、汉黄芩苷、峰9、峰11、峰15对应成分的含量变化均是导致样品质量差异的重要原因。结论该方法中建立的高效液相色谱指纹图谱可为双黄连片的质量评价提供参考。Objective To establish a high-performance liquid chromatography(HPLC)fingerprint of Shuanghuanglian Tablets,and to conduct cluster analysis and principal component analysis.Methods The determination was performed on Agilent Zorbax-C18(250 mm×4.6 mm,5μm)column with mobile phase consisted of acetonitrile-0.07%formic acid solution(gradient elution)at the flow rate of 0.9 mL/min.The detection wavelength was 320 nm for chlorogenic acid,l uteolin-7-glucoside,220 nm for forsythoside A,forsythin and 275 nm for baicalin,wogonoside and baicalein with 30℃column temperature.The injection volume was 10μL.Taken arctiin as reference peak,the HPLC fingerprints of 12 batches of Shuanghuanglian Tablets were determined,and the similarity in the 12 batches of samples was evaluated by Traditional Chinese Medicine Chromatographic Fingerprint Evaluation System(2012 edition)to determine the common peak.Cluster analysis and principal component analysis were performed by using SPSS 22.0 statistical software.Results The HPLC fingerprints of the 12 batch of Shuanghuanglian Tablets were established,and a total of 18 common peaks were calibrated.The method was validated by methodological investigation.The medicinal materials of the same origin could be grouped into one class.Through principal component analysis,accumulative contribution rate of 2 principal component factors was 92.242%.It was indicated that the content change of corresponding components of common peaks of No.9,11,15,chlorogenic acid,luteolin-7-glucoside,forsythoside A,forsythin,baicalin,wogonoside and baicalein in samples was an important reason for the quality difference of samples.Conclusion The established HPLC fingerprint can provide reference for quality evaluation of Shuanghuanglian Tablets.

关 键 词：双黄连片 高效液相色谱法 指纹图谱 聚类分析 主成分分析 质量控制 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]