高效液相色谱法测定决明子4种成分含量  

Determination of Four Active Ingredient in Cassiae Semen by HPLC

作　　者：申玉龙 徐达 金艳敏 刘勇[2] 王立伟 安丽娜 SHEN Yulong;XU Da;JIN Yanmin;LIU Yong;WANG Liwei;AN Lina(Chengde Yanfeng Pharmaceutical Group Co.,Ltd.,Chengde,Hebei,China 067600;Hebei Provincial People's Hospital,Shijiazhuang,Hebei,China 050051)

机构地区：[1]承德燕峰药业有限责任公司,河北承德067600 [2]河北省人民医院,河北石家庄050051

出　　处：《中国药业》2020年第9期134-136,共3页China Pharmaceuticals

摘　　要：目的建立同时测定决明子中4种成分含量的高效液相色谱(HPLC)法。方法色谱柱为迪马Dimonsil柱(150 mm×4.6mm,5μm),流动相为乙腈-甲醇-0.1%磷酸溶液(49∶7∶44,V/V/V),等度洗脱,柱温为30℃,检测波长为222 nm,流速为1.0 mL/min,进样量为10μL。结果进样量线性范围橙黄决明素为0.02464~0.2464μg(r=0.99999),大黄素为0.0174~0.174μg(r=0.99999),大黄酚为0.02956~0.2956μg(r=0.99999),大黄素甲醚为0.016~0.16μg(r=0.99998);平均回收率橙黄决明素、大黄素、大黄酚、大黄素甲醚分别为99.41%,99.51%,99.48%和99.24%,RSD分别为0.58%,0.82%,0.95%和0.90%(n=6)。结论该方法可用于决明子质量的综合评价。Objective To establish a simultaneous determination method of four active ingredient in cassiae semen by high-performance liquid chromatography(HPLC).Methods The chromatographic column was Dikma Dimonsil column(150 mm×4.6 mm,5μm).The contents were determined using a mixture of acetonitrile,methanol and 0.1%phosphoric acid(49∶7∶44,V/V/V)as the mobile phase by HPLC with equivalent elution.Results The methodological study showed that a good linear correlation existed in the range 0.02464-0.2464μg(r=0.99999)for aurantio-obtusin,0.0174-0.174μg(r=0.99999)for emodin,0.02956-0.2956μg(r=0.99999)for chrysophanol and 0.016-0.16μg(r=0.99998)for epoxine methyl ether,respectively.The average recoveries for aurantio-obtusin,emodin,chrysophanol and aurantio-obtusin were 99.41%,99.51%,99.48%and 99.24%,respectively(n=6).The RSDs were 0.58%,0.82%,0.95%and 0.90%(n=6),respectively.Conclusion This method can be used for the comprehensive evaluation on the quality of cassiae semen.

关 键 词：高效液相色谱法 决明子 橙黄决明素 大黄素 大黄酚 大黄素甲醚 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]