膜迷路积水对豚鼠听觉外周系统振动特性的影响  

Effect of endolymphatic hydrops on vibration characteristics of guinea pig auditory periphery system

作　　者：王延年 黄煜 张玲[2] 时海波[2] 蒋伟康[1] WANG Yannian;HUANG Yu;ZHANG Ling;SHI Haibo;JIANG Weikang(Institute of Vibration,Shock and Noise,Shanghai JiaoTong University,Shanghai 200240;Affiliated Sixth People’s Hospital of Shanghai Jiaotong University,Shanghai 200233)

机构地区：[1]上海交通大学振动冲击噪声研究所,上海200240 [2]上海交通大学附属第六人民医院,上海200233

出　　处：《声学学报》2020年第3期425-432,共8页Acta Acustica

基　　金：多学科交叉项目培育(医工)基金(YG2016ZD02)资助。

摘　　要：为探究梅尼埃病引起的膜迷路积水对豚鼠听觉系统振动特性的作用机制,通过药物注射建立了内耳膜迷路积水豚鼠模型模拟梅尼埃病病理,搭建了多普勒激光测振系统对健康和膜迷路积水豚鼠听觉系统的振动特性进行测试研究,得到了健康与膜迷路积水豚鼠的听觉系统振动特性,并通过对比明确了内耳膜迷路积水对豚鼠听觉系统振动特性的影响。实验结果表明,在测试频率范围内,离体豚鼠镫骨及圆窗膜的振动特性与活体豚鼠无明显差异,离体豚鼠听觉系统振动特性与输入声压无关,镫骨及圆窗膜的振动位移与输入声压具有明显的线性关系。膜迷路积水显著降低了豚鼠听觉系统镫骨及圆窗膜的振动幅度,在低频及高频区间尤为明显,在500 Hz处镫骨最高由11.64 nm降低至2.27 nm,圆窗膜由24.97 nm降低至5.05 nm。在10 kHz处,圆窗膜最高由0.45 nm降低至0.03 nm。同时膜迷路积水导致豚鼠耳蜗传递比普遍下降,最高在1 kHz和10 kHz处分别由2.82,2.91下降至1.46,0.28。To clarify the mechanism of endolymphatic hydrops caused by Meniere’s disease on vibration characteristics of guinea pig auditory periphery system.The endolymphatic hydrops model was established by injecting the drugs and the vibration characteristics of the healthy and endolymphatic hydrops guinea pig ear sound system were tested by a Doppler laser vibration measurement system.The influences of endolymphatic hydrops on the acoustic characteristics of the guinea pig hearing system were clarified by comparing the results of control group and endolymphatic hydrops group.The results showed that the isolated cochlea of guinea pig had obvious linear characteristics.There was no significant difference in the vibration characteristics of stape and round window membrane between isolated guinea pig cochlea and live guinea pig cochlea.Endolymphatic hydrops reduced the vibration amplitude of the stape and round window membrane,especially in the low frequency and high frequency range.The vibration amplitude of stape decreased from11.64 nm to 2.27 nm at 500 Hz,and the round window membrane decreased from 24.97 nm to 5.05 nm.At 10 kHz,the round window membrane decreased from 0.45 nm to 0.03 nm.At the same time,endolymphatic hydrops caused a general decrease in the cochlear transmission ratio of guinea pigs,which dropped from 2.82 to 1.46 at 1 kHz,and 2.91 to 0.28 at 10 kHz,respectively.

关 键 词：传递比 位移幅值 听觉系统 圆窗膜 KHZ 梅尼埃病 听觉外周系统 振动特性 

分 类 号：R764[医药卫生—耳鼻咽喉科]