毛竹(Phyllostachys edulis)PheGT8和PheGT16基因的克隆与表达分析  

Cloning and Expression Analysis of PheGT8 and PheGT16 Genes in Moso Bamboo(Phyllostachys edulis)

作　　者：高红艳 刘俊 宋笑龙 孔波 李雪平 Gao Hongyan;Liu Jun;SongXiaolong;Kong Bo;Li Xueping(Key Laboratory of Bamboo and Rattan Science and Technology,State Forestry Administration,International Center for Bamboo and Rattan,National Forestry and Grassland Administration,Beijing,100102)

机构地区：[1]国际竹藤中心,国家林业和草原局竹藤科学与技术重点开放实验室,北京100102

出　　处：《分子植物育种》2020年第9期2842-2851,共10页Molecular Plant Breeding

基　　金：国家林业局948项目(2014-4-74);“十三五”国家重点研发计划(2018YFD0600102)共同资助。

摘　　要：三螺旋转录因子在植物响应非生物胁迫过程中具有潜在的作用。本研究通过对毛竹(Phyllostachys edulis)中2个三螺旋转录因子基因进行生物信息学分析,并对其在不同胁迫条件下的表达水平进行研究,初步解析毛竹三螺旋转录因子基因的功能。本研究克隆得到2个毛竹三螺旋转录因子基因PheGT8和PheGT16,发现这两个基因在低温、干旱、盐胁迫以及脱落酸(abscisic acid,ABA)、吲哚-3-乙酸(indole-3-acetic acid,IAA)、水杨酸(salicylic acid,SA)和茉莉酸甲酯(methyl jasmonate,MeJA)处理时,表达均呈现了不同程度的上调或下调,表明这两个基因参与了毛竹应对非生物胁迫的应答过程,具有潜在的调控作用。Trihelix transcription factors have a potential role in plants in response to abiotic stresses.In this study,bioinformatics analysis was carried out on two trihelix genes of moso bamboo,and the expression levels of the trihelix genes under different abiotic stress conditions were studied to analyze preliminarily the function of the trihelix genes in moso bamboo.Two trihelix genes from moso bamboo,PheGT8 and PheGT16,were cloned.The expressions of the two genes were of a different degree up or down regulated under low temperature,drought,salt stress and different hormones(such as ABA,IAA,SA and MeJA)treatments,indicating that the two genes may be involved in the stress-resistance process of P.edulis and may have potential regulatory effects.

关 键 词：毛竹(Phyllostachys edulis) PheGT8 PheGT16 非生物胁迫 基因表达 

分 类 号：S795.7[农业科学—林木遗传育种]