转Cry2A基因抗虫甘蔗植株的培育  

Nurture of Pest Resistant Sugarcane by Genetic Transformation of Cry2A Gene

作　　者：王文治[1] 胡天娇 杨本鹏[1] 王俊刚[1] 冯小艳[1] 熊国如[1] 冯翠莲[1] 赵婷婷[1] 沈林波[1] 林拥军[3] 张树珍[1] Wang Wenzhi;Hu Tianjiao;Yang Benpeng;Wang Jungang;Feng Xiaoyan;Xiong Guoru;Feng Cuilian;Zhao Tingting;Shen Linbo;Lin Yongjun;Zhang Shuzhen(Institute of Tropical Bioscience and Biotechnology,Haikou,571101;College of Life Sciences,Nanjing Agricultural University,Nanjing,210095;College of Life Science and Technology of Huazhong Agricultural University,Wuhan,430070)

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101 [2]南京农业大学生命科学学院,南京210095 [3]华中农业大学生命科学技术学院,武汉430070

出　　处：《分子植物育种》2020年第9期2893-2898,共6页Molecular Plant Breeding

基　　金：中国热带农业科学院基本科研业务费国家自然科学基金培育项目(1630052019030);中国热带农业科学院基本科研业务费科技创新团队项目(19CXTD-33);现代农业产业技术体系建设专项资金(CARS-170301)共同资助。

摘　　要：Bt基因具有广谱杀虫性,培育转基因抗虫品种为甘蔗病虫害防治提供了新思路。为了检验Cry2A基因对甘蔗螟虫的防治效果,本研究构建了由玉米Ubi启动子驱动的Cry2A基因高效植物表达载体Cry2A-3300,利用农杆菌介导技术,对甘蔗胚性愈伤组织材料进行遗传转化,经过筛选培养及再生培养共得到46株抗性植株。对抗性植株进行PCR扩增检测,15株呈阳性;进一步利用RT-PCR,表明外源基因Cry2A在转录水平上得到有效表达。室内抗虫鉴定的结果表明Cry2A基因可有效抑制害虫的生长,这将有利于后期进行田间防治。Bt gene has a broad-spectrum insecticidal properties,so developing transgenic insect-resistant varieties provide a new idea for sugarcane pest control.In order to test the control effects of Cry2A gene,we constructed Cry2A-3300,a highly efficient plant expression vector of Cry2A gene driven by maize Ubi promoter.Then Agrobacterium-mediated technology was used for genetic transformation of embryonic callus materials of sugarcane,and 46 resistant plants were obtained through screening and regeneration.These plantlets were detected by PCR and 15 plantlets showed positive.Furthermore,RT-PCR analyses showed that the exogenous gene Cry2A was effectively expressed at the transcription level in these plantlets.The results of laboratory anti-insect identification showed that Cry2A gene could effectively inhibit the growth of pests.

关 键 词：甘蔗(Saccharum officinarum) Cry2A基因 遗传转化 甘蔗螟虫 

分 类 号：S435.66[农业科学—农业昆虫与害虫防治]