‘细叶’芒(Miscanthus sinensis’Variegatus’)四倍体诱导与表型分析  

Induction and Phenotypic Analysis of Tetraploid Miscanthus sinensis’Variegatus’

作　　者：吴海燕 岳跃森[2] 武菊英[2] 范希峰[2] 滕珂 斯琴巴特尔[1] 滕文军[2] Wu Haiyan;Yue Yuesen;Wu Juying;Fan Xifeng;Teng Ke;Sechenbaater;Teng Wenjun(College of Life Science and Technology,Inner Mongolia Normal University,Hohht,010022;Research&Development Center for Grass and Environment,Beijing Academy of Agriculture and Forestry Sciences,Beijing,100097)

机构地区：[1]内蒙古师范大学生命科学与技术学院,呼和浩特010022 [2]北京市农林科学院草业与环境研究发展中心,北京100097

出　　处：《分子植物育种》2020年第9期3018-3023,共6页Molecular Plant Breeding

基　　金：北京市农林科学院科技创新专项(KJCX20170110,KJCX20170207,KJCX20180101);北京市科技计划项目(171100007217001)共同资助。

摘　　要：本研究以观赏植物二倍体‘细叶’芒的幼穗为外植体诱导出愈伤组织,采用秋水仙素诱导处理获得其同源四倍体,分别通过流式细胞仪检测和根尖细胞染色体计数鉴定再生植株的倍性。进一步对四倍体‘细叶’芒的叶片气孔特征和植株表型特征分析,结果表明,与其二倍体亲本相比,四倍体叶片下表皮的气孔密度显著降低而气孔大小明显增大,四倍体植株的株高、分蘖和冠幅显著降低,但是叶片宽度比二倍体植株明显增加,而四倍体茎粗和叶长跟二倍体植株无明显差异。该研究获得的‘细叶’芒同源四倍体可为观赏植物新品种的培育提供新种质资源。In this study,callus was induced from young inflorescences of diploid Miscanthus sinensis’Variegatus’,an ornamental plant,and its autotetraploids were obtained by colchicine induction in this study.Flow cytometry and chromosomes in root tip cell were used to detect and identify the ploidy level of regenerated plants.Further,leaf stomatal characteristics and plant phenotypic characteristics of tetraploid Miscanthus sinensis’Variegatus’were analysised,and results showed that stomatal frequency reduced but stomatal size increased significantly in leaf epidermis of tetraploid compared with its diploid parent.Moreover,tetraploid showed increase in leaf width but decrease significantly in plant height,tiller and crown width than its diploid parent.But they had no difference in stem diameter and leaf length.Autotetraploids of Miscanthus sinensis’Variegatus’produced in this study provides new germplasm resources for cultivation of ornamental new varieties.

关 键 词：芒(Miscanthus sinensis) 观赏植物 秋水仙素 四倍体 

分 类 号：S578[农业科学—作物学]