大麦EMS突变体库构建和突变体筛选  

Construction of EMS-Treated Mutant Library and Mutant Phenotypic Analysis in Barley

作　　者：栾海业[1] 徐肖 陶红[1] 臧慧[1] 张英虎[1] 乔海龙[1] 杨红燕 陈健[1] 陈和[1] 沈会权[1] LUAN Haiye;XU Xiao;TAO Hong;ZANG Hui;ZHANG Yinghu;QIAO Hailong;YANG Hongyan;CHEN Jian;CHEN He;SHEN Huiquan(Jiangsu Coastal Area Institute of Agricultural Sciences,Yancheng,Jiangsu 224002,China)

机构地区：[1]江苏沿海地区农业科学研究所,江苏盐城224002

出　　处：《麦类作物学报》2020年第5期577-584,共8页Journal of Triticeae Crops

基　　金：国家大麦青稞产业技术体系项目(CARS-05);江苏省农业自主创新项目[CX(17)3056];江苏农业科技援藏-青稞种质资源鉴定及新品系选育项目[JATS(2018)139];上海市科技兴农项目(2019-02-08-00-08-F01109)。

摘　　要：为研究大麦重要功能基因并给大麦遗传育种提供新的种质资源,通过甲基磺酰乙酯(EMS)诱变处理优质、高抗黄花叶病大麦品种苏啤6号,从2400个M2代株系中筛选出152个突变株系,突变频率为6.33%。其中,幼苗习性突变株系36个,突变率为1.50%;叶部突变株系34个,突变率为1.42%;茎部突变株系33个,突变体率为1.38%;穗部突变株系22个,突变率为0.92%;成熟期和育性突变株系27个,突变率为1.13%。经过M3代验证,获得可稳定遗传的各类突变株系43个。In order to provide basic materials for studying the functional genomics and genetic breeding of barley,Supi 6 with a high quality and resistance to yellow mosaic disease was treated with the chemical mutagen ethyl methane sulfonate(EMS).A total of 152 mutants with significantly visible phenotypes were screened out from the 2400 M2 generation,with a mutation frequency of 6.33%.Of them,36 lines varied in seedling habits,with a mutation frequency of 1.50%;34 lines varied in leaf,with a mutation frequency of 1.42%;33 lines varied in stalk,with a mutation frequency of 1.38%;22 lines varied in spike,with a mutation frequency of 0.92%;27 lines varied in maturation stage and fertility,with a mutation frequency of 1.13%.In total,43 stably inherited lines were obtained by further agronomic trait investigation in M3 generation.

关 键 词：大麦 EMS诱变 突变体 

分 类 号：S512.3[农业科学—作物学] S330