Verification of Glyphosate Resistance, Lepidopteran Resistance and Wide Compatibility of Male Sterile Line E1C4008S in Rice  

作　　者：ZENG Qiang DENG Lihua HU Wenbin HE Xingjian MENG Qiucheng YU Jianghui LI Jinjiang WENG Lushui XIAO Guoying 

机构地区：[1]Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Hunan Rice Research Institute,Changsha 410125,China [4]The Sericultural Research Institute of Hunan Province,Changsha 410125,China [5]The Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Rice science》2020年第3期215-226,共12页水稻科学（英文版）

基　　金：funded by National Major Science and Technology Project on Breeding of New Genetically Modified Organisms(Grant No.2016ZX-08001003-001)。

摘　　要：Weeds and insect pests are two important biotic stresses resulting in yield loss in rice, and wide compatibility is the essential characteristic of breeding inter-subspecific hybrid rice. This study focused on glyphosate resistance, lepidopteran resistance and wide compatibility as well as identification of molecular and some agronomic characteristics of transgenic male sterile line E1 C4008 S. The results indicated that glyphosate resistance gene Epsps^# and lepidopteran resistance gene Cry1 ca^# were transferred into japonica wide compatibility male sterile line 4008 S by Agrobacterium-mediated method, and four independent transformation events named E1 C4008 S-1, E1 C4008 S-2, E1 C4008 S-3 and E1 C4008 S-4 were obtained, in which E1 C4008 S-3 and E1 C4008 S-4 were of single copy insertion. The EPSPS protein contents of E1 C4008 S-3 and E1 C4008 S-4 in different organs were significantly different both in descending order of leaf > stem > root, and ranged from 300.58 to 1410.69 μg/g at the tillering stage. The glyphosate tolerable concentration(dosage) of E1 C4008 S-3 and E1 C4008 S-4 reached at least 16 g/L(54.42 kg/hm^2), and the seeds of E1 C4008 S-4 can germinate normally on the medium containing 1 g/L glyphosate. The CRY1 C protein contents of E1 C4008 S-3 and E1 C4008 S-4 in different organs were significantly different both in descending order of leaf > stem > root, and ranged from 0.62 to 2.43 μg/g at the tillering stage. The larvae mortalities of rice leaf rollers fed on leaves of E1 C4008 S-3 and E1 C4008 S-4 for 5 d were 95.35% and 97.77%, respectively, while the average mortalities of silkworms fed with protein extracts from leaves of E1 C4008 S-3 and E1 C4008 S-4 reached 94.55% and 83.64%, respectively. The results suggested that wide compatibility and evaluated agronomic traits of E1 C4008 S-4 were not significantly changed by insertion of the exogenous genes. Overall, a novel male sterile germplasm with glyphosate resistance, lepidopteran resistance and wide compatibility was verified to be dev

关 键 词：RICE GLYPHOSATE RESISTANCE LEPIDOPTERAN RESISTANCE wide compatibility male STERILE line bioengineered crop 

分 类 号：S435.112[农业科学—农业昆虫与害虫防治] S451[农业科学—植物保护]