Association Mapping of Quantitative Trait Loci for Grain Size in Introgression Line Derived from Oryza rufipogon  

作　　者：Kashif HUSSAIN ZHANG Yingxing Workie ANLEY Aamir RIAZ Adil ABBAS MdHasanuzzaman RANI WANG Hong SHEN Xihong CAO Liyong CHENG Shihua 

机构地区：[1]Key Laboratory for Zhejiang Super Rice Research&State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China [2]Southern Rice Research Institute Thatta,Agriculture Research Wing,Government of Sindh,Sindh 73130,Pakistan [3]Department of Plant Sciences,University of Gondar,P.O.Box 196,Ethiopia [4]Bangladesh Institute of Nuclear Agriculture,Dhaka 2202,Bangladesh

出　　处：《Rice science》2020年第3期246-254,共9页水稻科学（英文版）

基　　金：supported by the National Key Research and Development Program(Grant No.2016YFD0101801);the Major Project of the Genetically Modified and National Key Transgenic Research Projects,China(Grant No.2016ZX08001-002);the National Natural Science Foundation of China(Grant No.31521064);Chinese Academy of Agricultural Sciences Innovation Project(CAASASTIP-2013-CNRRI);Fundamental Research Expenses of Central Public Welfare Research Institutions 2017 RG001-1.

摘　　要：Grain size is one of the critical agronomic traits governing grain yield and quality in rice.However,the underlying genetic mechanisms that control grain size in rice are poorly understood.We used an introgression line derived from Zhonghui 8015 and Oryza rufipogon Griff.This introgression line was evaluated under two different environmental conditions to dissect the quantitative trait loci controlling grain size.Genome-wide association study(GWAS)was performed using 28193 SNPs through a general linear model,and 56 significant SNPs on different loci associated with the 4 grain size traits were detected.Cloned genes including GS3 and q GL3 showed substantial effects on grain length and size.Seven new stable loci were identified with pleiotropic effects on grain size.Haplotype,gene expression analyses,combined gene-based associations,and functional annotations permitted the shortlisting of important dominant genes including GS3 and q GL3.

关 键 词：grain size ASSOCIATION mapping ORYZA SATIVA ORYZA RUFIPOGON quantitative TRAIT locus genome-wide ASSOCIATION analysis 

分 类 号：S511[农业科学—作物学]