甲硫脑啡肽对脂多糖作用下奶牛子宫内膜上皮细胞增殖的影响  

Effect of met-enkephalin on lipopolysaccharide-stimulated proliferation of bovine endometrial epithelial cell

作　　者：柴立昂 孙法壮 蔡和乐 李建基[1] 王亨[1] 李俊 崔璐莹 CHAI Li-ang;SUN Fa-zhuang;CAI He-le;LI Jian-ji;WANG Heng;LI Jun;CUI Lu-ying(Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses,College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China)

机构地区：[1]扬州大学兽医学院江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医学报》2020年第4期772-779,共8页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31802253,31672614);江苏省高校自然科学基因研究面上资助项目(17KJB230007);中国博士后科学基金资助项目(2018M632398);扬州大学2018年度江苏省研究生科研创新资助项目;江苏现代农业产业技术体系建设专项资金资助项目(JATS[2018]315);扬州大学优秀青年骨干教师资助项目;江苏高校优势学科建设工程资助项目(PAPD);江苏高校品牌专业建设工程资助项目(TAPP).

摘　　要：为探究甲硫脑啡肽(methionine enkephalin,MENK)对脂多糖(lipopolysaccharide,LPS)诱导的奶牛子宫内膜上皮细胞(BEEC)增殖的影响,利用CCK-8检测细胞增殖活性变化,荧光定量PCR检测细胞增殖相关基因(EGFR、VEGF、TGF-β3、FGF-2)表达,蛋白免疫印迹法检测Wnt/β-catenin通路关键因子、c-Myc和Cyclin D1表达。结果显示,LPS单独作用时,细胞活性增强(P<0.05),细胞增殖相关基因表达升高(P<0.05),β-catenin、c-Myc、Cyclin D1表达升高(P<0.05)。MENK能显著抑制LPS诱导的细胞活性(P<0.05)、细胞增殖相关因子及Wnt/β-catenin通路关键蛋白的表达(P<0.05);非选择性阿片受体拮抗剂纳洛酮可阻断MENK对细胞活性、细胞增殖相关因子和通路的抑制效应。MENK可抑制LPS诱导的奶牛子宫内膜上皮细胞的增殖作用,该机制很可能由阿片受体介导。To explore the effect of met-enkephalin(MENK)on the proliferation of bovine endometrial epithelial cells(BEEC)stimulated with lipopolysaccharide(LPS).The changes in cell viability were measured by CCK-8,the gene expressions of the cell proliferation factors were determined using qPCR,and the Wnt/β-catenin pathway were detected by Western blot.As a result,LPS treatment alone increased the cell viability,the mRNA expressions of proliferation factors,and protein expressions ofβ-catenin(P<0.05).The inhibitory effects of MENK were observed in the LPS-induced upregulations of cell viability,proliferation factors,andβ-catenin in BEEC(P<0.05),which was abolished by naloxone(NAL).MENK could inhibit the LPS induced cell proliferation,which was mediated by opioid receptors.

关 键 词：甲硫脑啡肽 奶牛子宫内膜上皮细胞 脂多糖 纳洛酮 细胞增殖 

分 类 号：S859.7[农业科学—临床兽医学]