獭兔线粒体基因组全序列的测定与分析  

Complete sequence determination and analysis of mitochondrial genome of Rex rabbit

作　　者：周娟[1] 李佳丽[1] 陈秋燃 刘明 陈阳[1] 吴信生[1] ZHOU Juan;LI Jia-li;CHEN Qiu-ran;LIU Ming;CHEN Yang;WU Xin-sheng(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国兽医学报》2020年第4期823-827,共5页Chinese Journal of Veterinary Science

基　　金：现代农业产业技术体系建设专项资金资助项目(CARS-43-A-1);江苏高校优势学科建设工程资助项目PAPD(2014-134)。

摘　　要：通过PCR扩增和TA克隆技术,获得獭兔线粒体基因组全序列,序列全长17361 bp,碱基组成分别为31.48%A、26.66%C、13.58%G、28.28%T。獭兔线粒体基因组包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个非编码控制区(D-loop)。在这37个编码的基因中,由轻链(L链)编码的基因9个,它们是ND6基因和8个tRNA基因,其余28个均由重链(H链)编码。其线粒体基因组成与其他兔属动物线粒体全基因组的排列顺序一致,表明兔属动物线粒体DNA进化上有较高的保守性。Complete mitochondrial genome sequence of the Rex rabbit was obtained by PCR sequencing and TA cloning,the entire mitochondrial genome of oryctolagus cuniculus is 17361 bp in length,which includes 13 protein coding genes(PCGs),22 tRNA genes,2 rRNA genes and 1 D-loop.9 of 37 coding genes are encoded by light chain,which includes 8 tRNA genes and ND6 gene.The other 28 coding genes are encoded by heavy chain.The composition of the nucleotides A,C,G,T are 31.48%,26.66%,13.58%,28.28%,respectively.The gene composition is the same as the mitochondrial genome organization of other species in lepus,which indicated that the mitochondrial DNA of lepus is evolutionarily highly conserved.

关 键 词：獭兔 线粒体基因组 序列分析 

分 类 号：S813.3[农业科学—畜牧学]