北柴胡地上部分正丁醇层的化学成分研究  

Chemical Constituents of N-Butanol Layer from the Overground Part of Bupleurum Chinese DC.

作　　者：黄金[1] 刘艳[1] 潘娟[1] 刘源[1] 许振鹏 杨炳友[1] 匡海学[1] HUANG Jin;LIU Yan;PAN Juan;LIU Yuan;XU Zhenpeng;YANG Bingyou;KUANG Haixue(Key Laboratory of Chinese Materia Medica of Ministry of Education,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学北药基础与应用研究教育部重点实验室,黑龙江哈尔滨150040

出　　处：《中医药学报》2020年第1期38-41,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81973440)。

摘　　要：目的:研究北柴胡地上部分正丁醇层的化学成分。方法:以70%乙醇提取北柴胡地上部分,并依次采用石油醚、乙酸乙酯、正丁醇进行萃取,利用硅胶、ODS、Sephadex LH-20等柱色谱及制备型HPLC对正丁醇层进行分离纯化,根据理化性质及核磁共振波谱数据鉴定化合物结构。结果:从北柴胡地上部分正丁醇部位中分离得到9个化合物,分别鉴定为核糖醇(1)、邻苯二甲酸二丁酯(2)、棕榈酸(3)、焦谷氨酸正丁酯(4)、3-methyl-but-2-en-1-yl β-D-glucopyranoside(5)、3-methylbutan-1-ol β-D-glucopyranoside(6)、(Z)-3-hexenyl-O-β-D-glucopyranoside(7)、1-(2'-deoxy-β-D-erythro-pentofuranosyl)-1H-1,2,4-triazone(8)、anoectochine(9)。结论:化合物4,8,9首次从伞形科中分离得到,化合物5~7为首次从该植物中分离得到。Objective:To study the chemical constituents of n-butanol layer from the overground part of Bupleurum Chinese DC..Methods:The aerial part of Bupleurum Chinese DC.was extracted by 70%ethanol,and then extracted by petroleum ether,ethyl acetate and n-butanol.The n-butanol layer is separated and purified by the process of silica gel,ODS,Sephadex LH-20 and HPLC(preparative type).The structures of the compounds were identified according to the physical and chemical properties and NMR data.Results:Nine compounds were isolated and identified as ribitol(1),dibutylphthalate(2),palmitic acid(3),n-butyl pyroglutamate(4),3-methyl-but-2-en-1-yl β-D-glucopyranoside(5),3-methylbutan-1-ol β-D-glucopyranoside(6),(Z)-3-hexenyl-O-β-D-glucopyranoside(7),1-(2'-deoxy-β-D-erythro-pentofuranosyl)-1H-1,2,4-tri azone(8)and anoectochine(9).Conclusion:Compounds 4,8 and 9 were isolated from the Apiaceae for the first time,also compounds 5?7 were isolated from this plant for the first time.

关 键 词：北柴胡 伞形科 地上部分 化学成分 

分 类 号：R284[医药卫生—中药学]