除草活性菌株极细链格孢菌(Alternaria tenuissima)发酵工艺研究  

Study on fermentation optimization of herbicidal activity strain Alternaria tenuissima

作　　者：朱海霞[1] 马永强[1] ZHU Hai-xia;MA Yong-qiang(Academy of Agriculture and Forestry Sciences of Qinghai University,State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Scientific Observing and Experimental Station of Crop Pest in Xining,Ministry of Agriculture,Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province,Xining 810016,China)

机构地区：[1]青海大学农林科学院,青海大学省部共建三江源生态与高原农牧业国家重点实验室,农业部西宁作物有害生物科学观测实验站,青海省农业有害生物综合治理重点实验室,青海西宁810016

出　　处：《甘肃农业大学学报》2020年第1期63-71,共9页Journal of Gansu Agricultural University

基　　金：青海省科技厅应用基础研究项目(2019-ZJ-7057).

摘　　要：【目的】探索一株防治藜、冬葵等阔叶杂草的生防菌株极细链格孢(Alternaria tenuissima)HZ-1的发酵工艺.【方法】采用液固双相发酵方式,通过单因素和正交设计试验,对该菌株固态发酵基质、最适碳氮源和固-液发酵条件进行研究.【结果】HZ-1最佳产孢的固态基质为麦麸,最适碳源为麦麸(40 mg/g),最适氮源为蛋白胨(50 mg/g).正交优化试验得出该菌最适固-液发酵条件为:培养基初始含水量为300 mg/g,适宜的接种量为0.3 mL/g,初始pH值7.4,最适温度为24.3℃,发酵最适时间为216 h.【结论】研究结果可为该极细链格孢菌菌株除草活性物质的分离及工业化生产提供依据.【Objective】To explore the fermentation process of bioherbicide strain Alternaria tenuissima HZ-1 which showed high virulence to broad-leaved weeds such as Chenopodium album and Malva crispa.【Method】The optimum carbon and nitrogen sources,solid substrate,culture conditions were studied by using a liquid-solid two-phase fermentation method with single-factor and orthogonal design experiments.【Result】The best spore-producing substrate was wheat bran,the optimal carbon source was wheat bran(40 mg/g),the optimum nitrogen source was peptone(50 mg/g).The optimum conditions for solid-liquid fermentation were obtained by orthogonal optimization of culture conditions:the initial moisture content of culture substrate was 300 mg/g,and the appropriate volume fraction of inoculation volume was 0.3 mL/g.The initial pH was 7.4,the optimum temperature was 24.3℃,the optimal cultural time was 216 hours.【Conclusion】The results provided a basis for isolation of herbicidal active substances and industrial production of the strain.

关 键 词：极细链格孢菌(Alternaria tenuissima) 固态发酵基质 固-液发酵条件 

分 类 号：S476[农业科学—农业昆虫与害虫防治]