下肢原发性慢性静脉功能不全与上皮间质转化之间的关系  

The relationship between primary chronic venous insufficiency of lower extremity and epithelial stromal transformation

作　　者：周亚东[1] 陈幸生[1] 官云彪[1] 薛明[1] ZHOU Ya-dong;CHEN Xing-sheng;GUAN Yun-biao;XUE Ming(Department of Vascular Surgery, Union Hospital of Fujian Medical University, Fuzhou 350001, China)

机构地区：[1]福建医科大学附属协和医院血管外科,福建福州350001

出　　处：《河北医科大学学报》2020年第4期418-421,共4页Journal of Hebei Medical University

摘　　要：目的探讨下肢原发性慢性静脉功能不全(primary chronic venous insufficiency,PCVI)与上皮间质转化之间的关系。方法选择PCVI患者67例,分为轻度PCVI组28例,中度PCVI组21例,重度PCVI组18例;另选择其他手术取大隐静脉作供体移植者16例作为对照组。采用半定量逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)和免疫印迹(Western blot)法检测大隐静脉标本中上皮钙黏附蛋白(E-cadherin)和波形蛋白(Vimentin)的表达情况。结果轻度PCVI组、中度PCVI组、重度PCVI组E-cadherin mRNA和蛋白平均灰度值低于对照组,中度PCVI组、重度PCVI组E-cadherin mRNA和蛋白平均灰度值低于轻度PCVI组,重度PCVI组E-cadherin mRNA和蛋白平均灰度值低于中度PCVI组(P<0.01)。正常对照组、轻度PCVI组、中度PCVI组、重度PCVI组中组织标本表达Vimentin mRNA和蛋白差异无统计学意义(P>0.05)。结论PCVI可能与上皮间质转化无关,但E-cadherin作为上皮细胞的表型标志,随临床严重程度升高而降低,可作为评估PCVI严重程度的一个指标。Objective To investigate the relationships between lower extremity primary chronic venous insufficiency(PCVI)and epithelial mesenchimal transition(EMT).Methods Sixty-seven patients with PCVI were classified into mild groups(n=28),moderate group(n=21),and severe group(n=18).Sixteen patients without PCVI were allocated to control group.Semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)and Western blot were used to detect the expression of E-cadherin and Vimentin in the varicose vein specimens.Results Expressions of E-cadherin mRNA and protein in mild group,moderate group and severe group were lower than those in control group.Between different PCVI groups,the expression of E-cadherin mRNA and protein in moderate group and severe group was lower than mild group(P<0.01).Similarly,expression of E-cadherin mRNA and protein in severe PCVI group were lower than those in moderate PCVI group.The Vimentin mRNA and protein expression levels in mild,moderate and severe group had no significant difference to control group(P>0.05).Conclusion The vascular remodeling of PCVI seems to have no relation with EMT.But the expression level of E-cadherin is decreased with the clinical severity of PCVI,therefore it may be an indicator when assessing severity of PCVI.

关 键 词：静脉功能不全 上皮间质转化 血管重塑 

分 类 号：R543.6[医药卫生—心血管疾病]