水稻染色体片段代换系Z745的鉴定及穗部性状QTL定位  

Identification of Chromosome Segment Substitution Line Z745 of Rice and QTL Mapping of Panicle Traits

作　　者：张治海 赵芳明[2] 罗洪发[1] 赵福胜[1] 潘清洁 杨旭东[1] 查仁明[1] ZHANG Zhihai;ZHAO Fangming;LUO Hongfa;ZHAO Fusheng;PAN Qingjie;YANG Xudong;ZHA Renming(College of Agriculture,Guizhou University,Guiyang 550025,China;Rice Research Institute,Southwest University/Key Laboratory of Biotechnology and Crop Quality Improvement,Ministry of Agriculture,Chongqing 400716,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]西南大学水稻研究所/农业部生物技术与作物品质改良重点开放实验室,重庆400716

出　　处：《河南农业科学》2020年第5期40-47,共8页Journal of Henan Agricultural Sciences

基　　金：贵州省科技厅项目(黔教合KY字[2015]333);中央高校基本科研业务费专项(XDJK2017A004)。

摘　　要：对水稻染色体片段代换系Z745进行染色体代换片段鉴定,并用以日本晴为背景亲本、水稻染色体片段代换系Z745为供体亲本杂交构建的次级F2群体定位水稻穗部性状QTL,以期发现新的穗部性状QTL。结果发现,Z745共含有17个代换片段,分布于第1、3—8、10和12染色体,代换片段总长度为26.1 Mb,平均代换长度为1.54 Mb;Z745的穗长、一次枝梗数、二次枝梗数和穗粒数均极显著高于日本晴;鉴定出21个穗部性状QTL,分布于其中9个代换片段上,位于第1、3—5、7—8、10和12共8条染色体上,其中,穗长QTL 4个(1、4和7染色体)、一次枝梗数QTL 4个(1、5、7和8染色体)、二次枝梗数QTL 6个(1、7、10和12染色体)、穗粒数QTL 7个(1、3、7、8、10染色体)。定位到的穗长QTL(qPL7-1、qPL7-2)、二次枝梗数QTL(qNSB7-1)和穗粒数QTL(qSPP1-1)已被报道,其余穗部性状QTL尚未报道。Chromosome segment identification of rice chromosome segment substitution line Z745 was conducted,and QTL mapping of panicle traits of secondary F2 population which was constructed by crossing with Nipponbare as the background parent and Z745 as the donor parent was studied,so as to find new QTL for panicle traits.The results showed that Z745 contained 17 substitution fragments,which were distributed on chromosomes 1,3—8,10 and 12.The replacement length was 26.1 Mb,and the average replacement length was 1.54 Mb.The panicle length,primary branch number,secondary branch number and grain number per panicle of Z745 were all significantly higher than those of Nipponbare.Twenty one QTLs of panicle traits were identified,which were distributed on nine substitutional segments,located on chromosomes 1,3—5,7—8,10 and 12.Among them,4 QTLs for panicle length were on chromosomes 1,4 and 7;4 QTLs for primary branch number were on chromosomes 1,5,7 and 8;6 QTLs for secondary branch number were on chromosomes 1,7,10 and 12;7 QTLs for grain number per panicle were on chromosomes1,3,7,8 and 10 respectively.The panicle length QTLs(qPL7-1,qPL7-2),secondary branch number QTL(qNSB7-1)and grain number per panicle QTL(qSPP1-1)located in this study had been reported,while the remaining ear trait QTLs had not been reported yet.

关 键 词：QTL定位 穗部性状 染色体片段代换系 水稻 

分 类 号：S511[农业科学—作物学]