莱氏绿僵菌Nr0815分生孢子在化学农药中的相容性  

Storage Compatibility of Metarhizium rileyi Strain Nr0815 Conidium in Chemical Pesticides

作　　者：杜广祖[1] 张小娇 罗艳 陈斌[1] 张立敏[1,2] DU Guangzu;ZHANG Xiaojiao;LUO Yan;CHEN Bin;ZHANG Limin(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;College of Internation,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学植物保护学院,云南昆明650201 [2]云南农业大学国际学院,云南昆明650201

出　　处：《河南农业科学》2020年第5期104-110,共7页Journal of Henan Agricultural Sciences

基　　金：国家重点研发计划项目(2018YFD0200703);国家自然科学基金项目(30860005,31660537);云南省农业联合项目青年基金项目(201701YC0024);云南省烟草公司科技计划项目(2014YN16);云南省冬季马铃薯无公害高产技术体系构建及应用项目(云财农[2013]364号)。

摘　　要：为明确莱氏绿僵菌菌株Nr0815分生孢子在常见化学农药中的相容性,室内观察了2种除草剂(68%草甘膦铵盐可溶粒剂、200 g/L百草枯水剂)、3种杀菌剂(75%百菌清可湿性粉剂、80%多菌灵可湿性粉剂、65%代森锌可湿性粉剂)和2种杀虫剂(24%灭多威可溶液剂、20%甲氰菊酯乳油)对莱氏绿僵菌菌株Nr0815分生孢子萌发、菌丝生长和产孢量的影响。结果表明,7种化学农药对莱氏绿僵菌菌株Nr0815分生孢子萌发、菌丝生长和产孢量均具有一定的抑制作用,抑制效果表现为杀菌剂>杀虫剂>除草剂。其中,除草剂以68%草甘膦铵盐可溶粒剂对分生孢子萌发率、菌丝生长抑制率和产孢量的影响最小,在最大推荐使用质量浓度(8000μg/mL)下,72 h时的孢子萌发率、菌丝生长抑制率和产孢量分别为(22.00±3.40)%、(72.79±2.49)%、(4.93±0.44)×108个/mL。除草剂杀菌剂杀虫剂中,莱氏绿僵菌菌株Nr0815分别与68%草甘膦铵盐可溶粒剂、80%多菌灵可湿性粉剂和20%甲氰菊脂乳油的相容性较好。In order to clarify the storage compatibility of conidia of Metarhizium rileyi strain Nr0815 in common chemical pesticides,the conidia germination,mycelia growth and sporulation quantity of M.rileyi strain Nr0815 affected by 2 species of herbicides(68%glyphosate ammonium soluble granule,200 g/L paraquat water agent),3 species of bactericides(75%chlorothalonil wettable powder,80%carbendazim wettable powder,65%dansen zinc wettable powder)and 2 species of insecticides(24%methomyl solution,20%fenpropathrin emulsifiable concentrate)were studied through indoor observation.The results showed that 7 species of chemical pesticides had certain inhibition effects on conidia germination,mycelia growth and sporulation quantity of M.rileyi strain Nr0815,and the order of inhibition from large to small was bactericide,insecticide and herbicide.Among them,effect on the conidia germination,mycelia growth inhinbition rate and conidia production quantity by 68%glyphosate ammonium soluble granule at 72 h was the least.It was respectively(22.00±3.40)%,(72.79±2.49)%and(4.93±0.44)×108 conidia per milliliter at the maximum recommended concentration(8000μg/mL).M.rileyi strain Nr0815 has good compatibility with 68%glyphosate ammonium soluble granule,80%carbendazim wettability powder and 20%fenpropathrin emulsifiable concentrate respectively in herbicides,bactericides and insecticides.

关 键 词：莱氏绿僵菌 相容性 杀虫剂 杀菌剂 除草剂 孢子萌发率 产孢量 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]