沃尔巴克氏体(Wolbachia)结合昆虫绝育技术控制白纹伊蚊种群  

Combining Wolbachia-based approach and sterile insect techniques eliminate Aedes albopictus population in field

作　　者：郑小英[1] 吴瑜[1] 张东京 洪晓月[2] 奚志勇 ZHENG Xiaoying;WU Yu;ZHANG Dongjing;HONG Xiaoyue;XI Zhiyong(Key Laboratory of Tropical Disease Control,Ministry of Education,Sun Yat-sen University,Guangzhou 510080,China;College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;Guangzhou Wolbaki Biotech Co.,Ltd.,Guangzhou 510663,China;Department of Microbiology and Molecular Gentis,Michigan State University,East Lansing,MI 48824,USA)

机构地区：[1]中山大学热带病防治研究教育部重点实验室,广东广州510080 [2]南京农业大学植物保护学院,江苏南京210095 [3]威佰昆生物科技有限公司,广东广州510663 [4]密歇根州立大学,美国东兰辛MI48824

出　　处：《南京农业大学学报》2020年第3期387-391,共5页Journal of Nanjing Agricultural University

基　　金：广东省引进人才创新科研团队计划项目(2011S009);教育部科学技术研究重点(重大)项目(311030);广州开发区科技领军人才项目(2013L-P116)。

摘　　要：白纹伊蚊(Aedes albopictus)是全球范围最具有侵略性的入侵物种之一,是中国大陆登革病毒和寨卡病毒等病原体的主要传播媒介。传统的蚊媒控制方法对白纹伊蚊的控制效果不理想。我们通过显微胚胎注射建立了携带三型沃尔巴克氏体(Wolbachia)菌型(wAlbA、wAlbB和wPip)的白纹伊蚊HC蚊株,应用昆虫不相容技术结合昆虫绝育技术(IIT-SIT),在选定的2个试验现场进行持续释放大规模生产的HC雄蚊,以压制目标区域的白纹伊蚊种群。研究结果显示:在2个试验现场基本清除白纹伊蚊的种群,证明IIT-SIT是一种高效、绿色环保的蚊媒控制工具,应用于登革热、寨卡病毒病等蚊媒疾病的传播控制切实可行,给蚊虫防控提供崭新的思路。Aedes albopictus is one of the most invasive mosquitos globally.It is the main vector of Dengue and Zika viruses over the mainland of China and traditional approaches are not effective in Ae.albopictus control.We created a triple-Wolbachia infection(the artificially transinfected wPip,native wAlbA and wAlbB)HC line of Ae.albopictus and releasing HC males that were factory-reared in two field sites by using combining incompatible and sterile insect technique(IIT-SIT).Results showed that IIT-SIT enabled near elimination of field populations of Ae.albopictus at two field sites.This successful field trial demonstrated the feasibility of using IIT-SIT as a new effective and green-environment friendly mosquito-borne control tool in blocking of dengue fever,Zika virus and other mosquito-borne diseases transmission.Our research provides a new idea for mosquito vector control.

关 键 词：昆虫绝育技术 昆虫不相容技术 沃尔巴克氏体 白纹伊蚊 

分 类 号：R384.1[医药卫生—医学寄生虫学]