X射线对人外周血ISG20L1基因表达的影响  

The investigation of ISG20L1 expression after X-ray exposure on the peripheral blood

作　　者：马娅 侯殿俊[2] 李洁清[2] 毛雪松[2] 李卫国[2] 朱伟[2] 方连英[2] 贾喜明 乔建维[2] MA Ya;HOU Dianjun;LI Jieqing;MAO Xuesong;LI Weiguo;ZHU Wei;FANG Lianying;JIA Ximing;QIAO Jianwei(China Institute of Atomic Energy,Beijing 102413 China;Institute of Radiation Medicine Shandong Academy of Medical Sciences Shandong First Medical University)

机构地区：[1]中国原子能科学研究院,北京102413 [2]山东省医学科学院放射医学研究所山东第一医科大学

出　　处：《中国辐射卫生》2019年第6期601-605,共5页Chinese Journal of Radiological Health

基　　金：山东省医药卫生科技发展计划项目(2016ws0519);山东省医学科学院医药卫生科技创新工程(2017-13-B)。

摘　　要：目的研究X射线诱导的人外周血ISG20L1基因表达变化,探究ISG20L1基因作为早期辐射生物标志物的可行性。方法以健康人群外周血作为研究对象,分别用0、0.5、1.0、2.0、4.0和8.0 Gy X射线照射EDTA抗凝外周血后培养0、4、8、24和48 h后,用RT-PCR技术检测基因转录水平,分析X射线照射外周血后的ISG20L1基因转录水平与辐射剂量之间的量效关系及时效关系;分析14名健康献血者的本底表达水平,分析基因表达的本底差异性。结果不同剂量照射组与对照组(0 Gy组)的基因表达水平在照射后0 h比较无显著性差异,而对照组样本随着培养时间的延长先下降随后上升,在去除ISG2L1基因的代谢动力学的影响后发现不同剂量点ISG20L1基因转录水平随着照后时间延长表达上调,各个照射剂量点的时间响应良好。分析不同时间点的剂量响应的时候发现,在照后4 h ISG20L1基因随着照射剂量的增加转录水平有所增加,但未能呈现良好剂量效应关系,照射后8 h、12 h和48 h在剂量范围为0~4 Gy时呈现出了良好的剂量效应关系,R2分别为0.9789,0.9083,0.9600,但当照射剂量增大为8 Gy时,ISG20L1基因转录水平反而下降。结论ISG20L1基因在0~4 Gy照射剂量范围内在照射后8 h以后呈现良好的量效关系,具有作为辐射生物标志物的潜力。Objective To investigate the expression changes of ISG20L1 gene after X-ray radiation on the peripheral blood ex vivo.Study on the feasibility of ISG20L1 gene as a biomarker of early radiation injury.Methods The peripheral blood from healthy person were exposed to 0,0.5,1.0,2.0,4.0,8.0 Gy X-ray radiation,after that cultured 0,4,8,24,48 h,the gene transcriptional levels were detected by RT-PCR.The dose-and time-effect relationships between the transcriptional level of ISG20L1 and X-ray doses were analyzed.The background expression level of 14 healthy donors was analyzed.Results The transcription level of ISG20L1 gene at different dose points were up-regulated with the culture time after irradiation.There was no significant difference in the transcription level of ISG20L1 gene at 0 h after irradiation compared to the control group.The transcription level of ISG20L1 increased with radiation dose at 4 h after irradiation.However,no dose-response relationship was determined.There was a good dose-response relationship beginning from 8 h after irradiation when the dose range was 0~4 Gy(R2 are 0.9789,0.9083,0.9600).The transcriptional level of ISG20L1 was found to be reduced after the dose>8 Gy.Conclusion Our study indicated that ISG20L1 gene correlated well with radiation doses after 8 h irradiation within the dose range of 0~4Gy,indicating that ISG20L1 is of potential for surrogating radiation damage.

关 键 词：ISG20L1基因 电离辐射 辐射生物标志物 

分 类 号：Q691[生物学—生物物理学]